Overview

  • Product name
  • Description
    Rabbit polyclonal to ERAB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A 17 amino acid synthetic peptide (near the N terminal) (Human) conjugated to KLH.

Properties

Applications

Our Abpromise guarantee covers the use of ab17298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 27 kDa.
ELISA 1/10000 - 1/100000.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD).
  • Tissue specificity
    Expressed in normal tissues but is overexpressed in neurons affected in AD.
  • Involvement in disease
    Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) [MIM:300438]. MHBD deficiency leads to neurological abnormalities, including psychomotor retardation, and, in virtually all patients, loss of mental and motor skills.
    Defects in HSD17B10 are the cause of mental retardation syndromic X-linked type 10 (MRXS10) [MIM:300220]. MRXS10 is characterized by mild mental retardation, choreoathetosis and abnormal behavior.
    A chromosomal microduplication involving HSD17B10 and HUWE1 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.
  • Sequence similarities
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 17 beta hydroxysteroid dehydrogenase 10 antibody
    • 17 beta hydroxysteroid dehydrogenase type 10 antibody
    • 17-beta-HSD 10 antibody
    • 17-beta-hydroxysteroid dehydrogenase 10 antibody
    • 17b HSD10 antibody
    • 3 hydroxy 2 methylbutyryl CoA dehydrogenase antibody
    • 3 hydroxyacyl CoA dehydrogenase type 2 antibody
    • 3 hydroxyacyl CoA dehydrogenase type II antibody
    • 3-hydroxy-2-methylbutyryl-CoA dehydrogenase antibody
    • 3-hydroxyacyl-CoA dehydrogenase type II antibody
    • 3-hydroxyacyl-CoA dehydrogenase type-2 antibody
    • AB binding alcohol dehydrogenase antibody
    • ABAD antibody
    • Ads9 antibody
    • Amyloid beta binding polypeptide antibody
    • Amyloid beta peptide binding alcohol dehydrogenase antibody
    • Amyloid beta peptide binding protein antibody
    • Amyloid beta peptide binding protein antibody
    • CAMR antibody
    • DUPXp11.22 antibody
    • Endoplasmic Reticulum Amyloid Binding Protein antibody
    • Endoplasmic reticulum associated amyloid beta peptide binding protein antibody
    • Endoplasmic reticulum-associated amyloid beta-peptide-binding protein antibody
    • ER associated amyloid beta-binding protein antibody
    • ERAB antibody
    • HADH 2 antibody
    • HADH2 antibody
    • HCD 2 antibody
    • HCD2 antibody
    • HCD2_HUMAN antibody
    • Hsd17b10 antibody
    • Hydroxyacyl CoA Dehydrogenase type II antibody
    • Hydroxyacyl Coenzyme A dehydrogenase type II antibody
    • Hydroxysteroid (17 beta) dehydrogenase 10 antibody
    • Mental retardation X linked syndromic 11 antibody
    • MHBD antibody
    • Mitochondrial L3 Hydroxyacyl CoA Dehydrogenase antibody
    • Mitochondrial ribonuclease P protein 2 antibody
    • Mitochondrial RNase P protein 2 antibody
    • MRPP2 antibody
    • MRX17 antibody
    • SCHAD antibody
    • SDR5C1 antibody
    • Short chain dehydrogenase/reductase family 5C member 1 antibody
    • Short chain L 3 hydroxyacyl CoA dehydrogenase type 2 antibody
    • Short chain type dehydrogenase/reductase XH98G2 antibody
    • Short-chain type dehydrogenase/reductase XH98G2 antibody
    • Type 10 17b HSD antibody
    • Type 10 17beta hydroxysteroid dehydrogenase antibody
    • Type II HADH antibody
    • XH98G2 antibody
    see all

References

ab17298 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for getting back to me with details of your technical approach. Further to our telephone conversation I have searched the literature and I have determined that Calreticulin antibody - ER Marker (ab4) was used in the following publication; Luo X et al. C1q-calreticulin induced oxidative neurotoxicity: relevance for the neuropathogenesis of Alzheimer's disease. J Neuroimmunol 135:62-71 (2003). PubMed: 12576225. In this publication it details the approach adopted for the staining of rat cortical neurons. I consider this a good approach for the use of this antibody. In the methodology they detail; 2.5. Immunofluorescence staining of CaR To visualize CaR, RCN were cultured in 24-well plates and then fixed with 2% paraformaldehyde and 120 mM sucrose in phosphate-buffered saline (PBS) for 10 min at room temperature. After quenching paraformaldehyde with 0.1 M glycine in PBS, the samples were blocked with 50% goat serum, 1% bovine serum albumin (BSA) in PBS (blocking buffer) for 40 min. Cells were subsequently incubated with a rabbit polyclonal antibody against CaR in blocking buffer for 40 min at room temperature. After several washes with PBS, cells were incubated with Alexa-594 (red fluorescence)-conjugated goat anti-rabbit IgG (1:50 dilution, preabsorbed IgG for multiple staining, Molecular Probes) for 30 min. After washes with PBS, cells were mounted with Vectashield (Vector Laboratories, Burlingame, CA) and coverslip. Whilst the approach that you have adopted is similar with respect to the the blocking and permeabilisation steps I consider the duration of fixation that you are adopting too long. As previously discussed with my colleague I would like to recommend a fixation period of 10-15 minutes following the approach above. Unfortunately we do not have any publications that have applied ERAB antibody - Mitochondrial Marker (ab17298). However, once again I would like to suggest that you try applying this antibody following milder fixation duration of 2% for 10 minutes. Should this not improve the results that you have been obtaining please get back in touch with me.

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Answer

Thank you for your enquiry and for completing our technical questionnaire so comprehensively. I am sorry to hear that you have been having difficulties with this antibody. I have read your questionnaire and I have a few comments. ERAB associates with the endoplasmic reticulum and therefore whilst the other antibodies that you have been using may work well under these conditions I feel that this antibody may require some optimisation with respect to the permeabilization and fixation stages. You are using Saponin which serves to permeabilise the cells but I would like to suggest that you try using the slightly more aggressive 0.25% Triton. This may facilitate the accessibility of the antibody. Furthermore you are performing fixation for 30 minutes in 4% formaldehyde. I suggest that you try reducing the length of fixation to 10-15 minutes as the fixation may be compromising the binding of the epitope by occluding the antibody. I would also like to suggest an overnight incubation of the antibody. This is sometimes necessary to obtain good staining. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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