Overview

  • Product name
    Anti-ERAB antibody [EPR11839(B)]
    See all ERAB primary antibodies
  • Description
    Rabbit monoclonal [EPR11839(B)] to ERAB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human ERAB (UniProt Q99714).

  • Positive control
    • Jurkat, 293T, K562 and HeLa cell lysates; Human brain and kidney tissues; HeLa cells; K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 27 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD).
    • Tissue specificity
      Expressed in normal tissues but is overexpressed in neurons affected in AD.
    • Involvement in disease
      Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) [MIM:300438]. MHBD deficiency leads to neurological abnormalities, including psychomotor retardation, and, in virtually all patients, loss of mental and motor skills.
      Defects in HSD17B10 are the cause of mental retardation syndromic X-linked type 10 (MRXS10) [MIM:300220]. MRXS10 is characterized by mild mental retardation, choreoathetosis and abnormal behavior.
      A chromosomal microduplication involving HSD17B10 and HUWE1 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.
    • Sequence similarities
      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Cellular localization
      Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • 17 beta hydroxysteroid dehydrogenase 10 antibody
      • 17 beta hydroxysteroid dehydrogenase type 10 antibody
      • 17-beta-HSD 10 antibody
      • 17-beta-hydroxysteroid dehydrogenase 10 antibody
      • 17b HSD10 antibody
      • 3 hydroxy 2 methylbutyryl CoA dehydrogenase antibody
      • 3 hydroxyacyl CoA dehydrogenase type 2 antibody
      • 3 hydroxyacyl CoA dehydrogenase type II antibody
      • 3-hydroxy-2-methylbutyryl-CoA dehydrogenase antibody
      • 3-hydroxyacyl-CoA dehydrogenase type II antibody
      • 3-hydroxyacyl-CoA dehydrogenase type-2 antibody
      • AB binding alcohol dehydrogenase antibody
      • ABAD antibody
      • Ads9 antibody
      • Amyloid beta binding polypeptide antibody
      • Amyloid beta peptide binding alcohol dehydrogenase antibody
      • Amyloid beta peptide binding protein antibody
      • Amyloid beta peptide binding protein antibody
      • CAMR antibody
      • DUPXp11.22 antibody
      • Endoplasmic Reticulum Amyloid Binding Protein antibody
      • Endoplasmic reticulum associated amyloid beta peptide binding protein antibody
      • Endoplasmic reticulum-associated amyloid beta-peptide-binding protein antibody
      • ER associated amyloid beta-binding protein antibody
      • ERAB antibody
      • HADH 2 antibody
      • HADH2 antibody
      • HCD 2 antibody
      • HCD2 antibody
      • HCD2_HUMAN antibody
      • Hsd17b10 antibody
      • Hydroxyacyl CoA Dehydrogenase type II antibody
      • Hydroxyacyl Coenzyme A dehydrogenase type II antibody
      • Hydroxysteroid (17 beta) dehydrogenase 10 antibody
      • Mental retardation X linked syndromic 11 antibody
      • MHBD antibody
      • Mitochondrial L3 Hydroxyacyl CoA Dehydrogenase antibody
      • Mitochondrial ribonuclease P protein 2 antibody
      • Mitochondrial RNase P protein 2 antibody
      • MRPP2 antibody
      • MRX17 antibody
      • SCHAD antibody
      • SDR5C1 antibody
      • Short chain dehydrogenase/reductase family 5C member 1 antibody
      • Short chain L 3 hydroxyacyl CoA dehydrogenase type 2 antibody
      • Short chain type dehydrogenase/reductase XH98G2 antibody
      • Short-chain type dehydrogenase/reductase XH98G2 antibody
      • Type 10 17b HSD antibody
      • Type 10 17beta hydroxysteroid dehydrogenase antibody
      • Type II HADH antibody
      • XH98G2 antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling ERAB with purified ab167410 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • ab167410 showing +ve staining in Human heart.
    • ab167410 showing +ve staining in Human gastric adenocarcinoma.
    • ab167410 showing +ve staining in Human endometrial carcinoma.
    • ab167410 showing +ve staining in Human normal liver.
    • All lanes : Anti-ERAB antibody [EPR11839(B)] (ab167410) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 27 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ERAB with ab167410 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERAB with ab167410 at 1/50 dilution.
    • Immunofluorescent analysis of HeLa cells labeling ERAB with ab167410 at 1/100 dilution. DAPI nuclear staining (blue).
    • Flow cytometric analysis of permeabilized K562 cells labeling ERAB with ab167410 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    References

    ab167410 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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