Overview

  • Product name

  • Description

    Goat polyclonal to ERAP2
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Peptide with sequence C-

    QYLKKFSYRNAKND

    , corresponding to internal sequence amino acids 480-493 of human ERAP2 (NP_071745.1).

  • Positive control

    • Human spleen lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 110 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      ERAP2 is an aminopeptidase that plays a central role in peptide trimming. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP2 to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II. ERAP2 preferentially hydrolyzes the basic residues Arg and Lys.
    • Cellular localization

      Endoplasmic reticulum membrane; Single-pass type II membrane protein.
    • Database links

    • Alternative names

      • Endoplasmic reticulum aminopeptidase 2 antibody
      • L RAP antibody
      • Leukocyte derived arginine aminopeptidase antibody
      • LRAP antibody

    Images

    • Anti-ERAP2 antibody (ab82142) at 0.5 µg/ml + human spleen lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 110 kDa
      Observed band size: 110 kDa
      Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 hour


      An additional band of unknown identity was consistently observed at 50 kDa. This band was successfully blocked by incubation with the immunizing peptide.

    References

    ab82142 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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