Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab82142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 110 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceERAP2 is an aminopeptidase that plays a central role in peptide trimming. Major histocompatibility complex (MHC) class I molecules rely on aminopeptidases such as ERAP2 to trim precursors to antigenic peptides in the endoplasmic reticulum (ER) following cleavage in the cytoplasm by tripeptidyl peptidase II. ERAP2 preferentially hydrolyzes the basic residues Arg and Lys.
Cellular localizationEndoplasmic reticulum membrane; Single-pass type II membrane protein.
- Endoplasmic reticulum aminopeptidase 2 antibody
- L RAP antibody
- Leukocyte derived arginine aminopeptidase antibody
- LRAP antibody
Anti-ERAP2 antibody (ab82142) at 0.5 µg/ml + human spleen lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 hour
An additional band of unknown identity was consistently observed at 50 kDa. This band was successfully blocked by incubation with the immunizing peptide.
ab82142 has not yet been referenced specifically in any publications.