Overview

  • Product name
    Anti-ErbB 2 antibody [CB11]
    See all ErbB 2 primary antibodies
  • Description
    Mouse monoclonal [CB11] to ErbB 2
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-FoFr, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a site on the internal domain of the C-erbB-2 oncoprotein.

  • Positive control
    • Adenocarcinoma of the breast
  • General notes


    C-erbB-2 oncogene codes for a membrane protein, Neu protein. In several studies the amplification of C-erbB-2 oncogene in carcinomas has been reported. Overexpression of the Neu oncogene protein can be demonstrated by this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab8054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with acetone, 10 minutes at room temperature.
IHC-P 1/5 - 1/10.
WB 1/1000. PubMed: 17459787

Target

  • Function
    Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
    In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
  • Tissue specificity
    Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
  • Involvement in disease
    Hereditary diffuse gastric cancer
    Glioma
    Ovarian cancer
    Lung cancer
    Gastric cancer
    Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
  • Cellular localization
    Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    • C erb B2/neu protein antibody
    • CD340 antibody
    • CD340 antigen antibody
    • Cerb B2/neu protein antibody
    • CerbB2 antibody
    • Erb b2 receptor tyrosine kinase 2 antibody
    • ERBB2 antibody
    • ERBB2_HUMAN antibody
    • HER 2 antibody
    • HER 2/NEU antibody
    • HER2 antibody
    • Herstatin antibody
    • Human epidermal growth factor receptor 2 antibody
    • Metastatic lymph node gene 19 protein antibody
    • MLN 19 antibody
    • MLN19 antibody
    • NEU antibody
    • NEU proto oncogene antibody
    • Neuro/glioblastoma derived oncogene homolog antibody
    • Neuroblastoma/glioblastoma derived oncogene homolog antibody
    • NGL antibody
    • p185erbB2 antibody
    • Proto-oncogene c-ErbB-2 antibody
    • Proto-oncogene Neu antibody
    • Receptor tyrosine-protein kinase erbB-2 antibody
    • TKR1 antibody
    • Tyrosine kinase type cell surface receptor HER2 antibody
    • Tyrosine kinase-type cell surface receptor HER2 antibody
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
    • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
    • V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
    • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
    see all

References

This product has been referenced in:
  • Baiocchi GL  et al. Recurrence in node-negative advanced gastric cancer: Novel findings from an in-depth pathological analysis of prognostic factors from a multicentric series. World J Gastroenterol 23:8000-8007 (2017). Read more (PubMed: 29259375) »
  • Liu W  et al. ERBB2 Overexpression Establishes ERBB3-Dependent Hypersensitivity of Breast Cancer Cells to Withaferin A. Mol Cancer Ther 15:2750-2757 (2016). Read more (PubMed: 27474152) »
See all 14 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your enquiry.

I can confirm that ErbB2 is known to be expressed in breast issue and highly expressed in many breast cancers. Breast tumor tissue would therefore be a positive control to consider. You may want to do consider some further literature searching for more information:

http://www.ncbi.nlm.nih.gov/pubmed/1977679

We have some breast tumor tissue slides available:

ab4697 Breast tumor (human): ductal carcinoma (invasive) tissue slides
https://www.abcam.com/index.html?datasheet=4697 (or use the following: https://www.abcam.com/index.html?datasheet=4697).

The Unigene EST expression profile indicates it is expressed in many other tissues aswell:

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.446352

We have many slides available, please see the following link for a list of the slides we sell:

https://www.abcam.com/index.html?c=2300

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question
Answer

Thanks for your phone calls and for letting us know about the trouble with these antibodies. Your credit note ID is ***. I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. Please let me know if there is anything else that we can do for you. I wish you the best of luck with your research!

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Answer

Thank you for your enquiry and your interest in our products. We would like to confirm that this antibody recognizes ErbB2 and it has been tested and characterised for IHC (both on frozen and on formalin-fixed paraffin-embedded sections) and for IF. We have actually published one review on-line regarding its suitability for IF, please take a look at it. For details on IHC (antigen retrieval), please click on the Resources section on the top panel of the Abcam homepage, you might find some useful information there. As you probably know, recommended positive control for this antibody is breast adenocarcinoma. If you need anything further or any help then please let us know.

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Answer

Thank you for your enquiry. Ab2429 is the ErbB2 peptide used to generate Ab2428 - Rabbit polyclonal to ErbB2. It is a human C terminal synthetic peptide with the sequence of: KGTPTAENPEYLGLDVPV For ab8054, we don't see the antigen. If you have any additional questions, please contact us again.

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