Western blot abreview for Anti-ErbB 2 antibody [EPR19547-12]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (mammary epithelial)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
40 µg
Specification
mammary epithelial
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight
Dilution
1/1000

Secondary antibody

Dilution
1/4000
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
MCF-7 cells.
Exposure
10 second(s)
Bands
Specific: 180 kDa Non-specific: 140, 120, 90 kDa
Positive control
Cell lines: SKBR-3 and HCC199.

Flavia Ghiraldini

Verified customer

Submitted Sep 15 2016

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