Immunocytochemistry/ Immunofluorescence abreview for Anti-ErbB 2 antibody [EPR19547-12]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Derived Breast Cancer Cells)
Permeabilization
Yes - 0.5% Triton for 20 minutes
Specification
Mouse Derived Breast Cancer Cells
Blocking step
3% BSA + 5% Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C
Fixative
Paraformaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 26°C · Diluent: Blocking Buffer
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Goat Anti Rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Additional data

Additional Notes
The antibody works very well at the dilution of 1:500. In my system, dilutions below 1:500 were too strong. Picture shows Green Channel (ErbB 2) and Blue Channel (Hoechst Staining)

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Submitted Nov 20 2017

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