Anti-ErbB2 / HER2 antibody - N-terminal (ab219062)
Key features and details
- Rabbit polyclonal to ErbB2 / HER2 - N-terminal
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-ErbB2 / HER2 antibody - N-terminal
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit polyclonal to ErbB2 / HER2 - N-terminal -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other human proteins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Dog, Pig, Monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Human ErbB2/ HER2 (N terminal). (13 amino acid peptide from N-terminal extracellular domain).
Database link: P04626 -
Positive control
- Human prostate tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.099% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment with Proteinase K. |
Notes |
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IHC-P
Use a concentration of 5 - 10 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment with Proteinase K. |
Target
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Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
Tissue specificity
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
Involvement in disease
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
Cellular localization
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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Database links
- Entrez Gene: 505709 Cow
- Entrez Gene: 403883 Dog
- Entrez Gene: 101140885 Gorilla
- Entrez Gene: 100054739 Horse
- Entrez Gene: 2064 Human
- Entrez Gene: 100516641 Pig
- Omim: 164870 Human
- SwissProt: O18735 Dog
see all -
Alternative names
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab219062 has not yet been referenced specifically in any publications.