Anti-ErbB 2 (phospho Y877) antibody [EP2324Y] (ab108371)


  • Product name
    Anti-ErbB 2 (phospho Y877) antibody [EP2324Y]
    See all ErbB 2 primary antibodies
  • Description
    Rabbit monoclonal [EP2324Y] to ErbB 2 (phospho Y877)
  • Host species
  • Specificity
    ab108371 detects ErbB 2 phosphorylated at tyrosine 877.
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ErbB 2 (phospho Y877). The exact sequence is proprietary.

  • Positive control
    • HeLa cell lysate, SKBR-3 cell lysate, Human breast ductal infiltrating carcinoma tissue, SKBR-3 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab108371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 138 kDa.
IHC-P 1/250 - 1/500. Perform antigen retrieval before commencing with IHC staining protocol
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
      In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
    • Tissue specificity
      Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
    • Involvement in disease
      Hereditary diffuse gastric cancer
      Ovarian cancer
      Lung cancer
      Gastric cancer
      Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
    • Cellular localization
      Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
    • Information by UniProt
    • Database links
    • Alternative names
      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
      • C erb B2/neu protein antibody
      • CD340 antibody
      • CD340 antigen antibody
      • Cerb B2/neu protein antibody
      • CerbB2 antibody
      • Erb b2 receptor tyrosine kinase 2 antibody
      • ERBB2 antibody
      • ERBB2_HUMAN antibody
      • HER 2 antibody
      • HER 2/NEU antibody
      • HER2 antibody
      • Herstatin antibody
      • Human epidermal growth factor receptor 2 antibody
      • Metastatic lymph node gene 19 protein antibody
      • MLN 19 antibody
      • MLN19 antibody
      • NEU antibody
      • NEU proto oncogene antibody
      • Neuro/glioblastoma derived oncogene homolog antibody
      • Neuroblastoma/glioblastoma derived oncogene homolog antibody
      • NGL antibody
      • p185erbB2 antibody
      • Proto-oncogene c-ErbB-2 antibody
      • Proto-oncogene Neu antibody
      • Receptor tyrosine-protein kinase erbB-2 antibody
      • TKR1 antibody
      • Tyrosine kinase type cell surface receptor HER2 antibody
      • Tyrosine kinase-type cell surface receptor HER2 antibody
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
      • V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
      see all


    • All lanes : Anti-ErbB 2 (phospho Y877) antibody [EP2324Y] (ab108371) at 1/1000 dilution

      Lane 1 : A431 cell lysate - untreated
      Lane 2 : A431 cell lysate - treated with Epidermal Growth Factor (EGF)

      Lysates/proteins at 10 µg per lane.

      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/100000 dilution

      Predicted band size: 138 kDa
      Observed band size: 180 kDa (why is the actual band size different from the predicted?)

      Exposure time: 1 minute

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry analysis of A431 (human epidermoid carcinoma) untreated/treated with 200 ng/ml EGF for 15 minutes labeling ErbB 2 with purified ab108371 at 1/20. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Dot blot analysis of ErbB 2 (pY877) peptide (Lane 1) and ErbB2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y877) with ab108371 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • All lanes : Anti-ErbB 2 (phospho Y877) antibody [EP2324Y] (ab108371) at 1/500 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : EGF treated HeLa cell lysate
      Lane 3 : SKBR-3 cell lysate
      Lane 4 : EGF treated SKBR-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 138 kDa

    • ab108371, at 1/250, staining Human ErbB 2 (phospho Y877) in Human breast ductal infiltrating carcinoma tissue by immunohistochemistry.
    • ab108371, at 1/50 dilution, staining Human ErbB 2 (phospho Y877) in SKBR-3 cells by immunofluorescence.


    This product has been referenced in:
    • Oh DY  et al. Precision medicine approaches to lung adenocarcinoma with concomitant MET and HER2 amplification. BMC Cancer 17:535 (2017). Read more (PubMed: 28806950) »
    • Mo T  et al. Effect of Fu-Zheng-Xiao-Liu Granules on Expression of Human Epidermal Growth Factor Receptor 2 (HER-2) and Proliferation and Apoptosis of Breast Cancer Cell Line SKBR-3. Med Sci Monit 22:5068-5073 (2016). Read more (PubMed: 28008166) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting Abcam. The anti-ErbB 2 (phospho Y877) antibody [EP2324Y], ab108371 will not react with mouse samples. However ab47262, will work in mouse samples and detects Y877 phosphorylated residue. If there is anything else I can do, plea...

    Read More


    Sign up