Recombinant
RabMAb

Recombinant Anti-ErbB 2 (phospho Y877) antibody [EPR2324Y] (ab75873)

Overview

  • Product name

    Anti-ErbB 2 (phospho Y877) antibody [EPR2324Y]
    See all ErbB 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2324Y] to ErbB 2 (phospho Y877)
  • Host species

    Rabbit
  • Specificity

    ab75873 detects ErbB 2 phosphorylated at tyrosine 877.
  • Tested applications

    Suitable for: Dot blot, WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ErbB 2 (phospho Y877). The exact sequence is proprietary.

  • Positive control

    • HeLa and SKBR3 cell lysates treated with EGF. SKBR3 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab75873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 138 kDa).
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
      In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
    • Tissue specificity

      Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
    • Involvement in disease

      Hereditary diffuse gastric cancer
      Glioma
      Ovarian cancer
      Lung cancer
      Gastric cancer
      Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
    • Cellular localization

      Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
    • Information by UniProt
    • Database links

    • Alternative names

      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
      • C erb B2/neu protein antibody
      • CD340 antibody
      • CD340 antigen antibody
      • Cerb B2/neu protein antibody
      • CerbB2 antibody
      • Erb b2 receptor tyrosine kinase 2 antibody
      • ErbB-2 proto-oncogene antibody
      • ERBB2 antibody
      • ERBB2_HUMAN antibody
      • HER 2 antibody
      • HER 2/NEU antibody
      • HER2 antibody
      • Herstatin antibody
      • Human epidermal growth factor receptor 2 antibody
      • Metastatic lymph node gene 19 protein antibody
      • MLN 19 antibody
      • MLN19 antibody
      • NEU antibody
      • NEU proto oncogene antibody
      • Neuro/glioblastoma derived oncogene homolog antibody
      • Neuroblastoma/glioblastoma derived oncogene homolog antibody
      • NGL antibody
      • p185erbB2 antibody
      • Proto-oncogene c-ErbB-2 antibody
      • Proto-oncogene Neu antibody
      • Receptor tyrosine-protein kinase erbB-2 antibody
      • TKR1 antibody
      • Tyrosine kinase type cell surface receptor HER2 antibody
      • Tyrosine kinase-type cell surface receptor HER2 antibody
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
      • V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
      see all

    Images

    • All lanes : Anti-ErbB 2 (phospho Y877) antibody [EPR2324Y] (ab75873) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with EGF
      Lane 3 : SKBR3 cell lysate, untreated
      Lane 4 : SKBR3 cell lysate, treated with EGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 138 kDa
      Observed band size: 180 kDa
      why is the actual band size different from the predicted?

    • Dot blot analysis of ErbB 2 (pY877) peptide (Lane 1) and ErbB 2 non-phospho peptide (Lane 2) labelling ErbB 2 (phospho Y877) with ab75873 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Flow Cytometry analysis of A431 (human epidermoid carcinoma) untreated/treated with 200 ng/ml EGF for 15 minutes labeling ErbB 2 with unpurified ab75873 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • ab75873 at 1/50 dilution staining ErbB 2 in SKBR3 cells by Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Zahari MS  et al. Activating Mutations in PIK3CA Lead to Widespread Modulation of the Tyrosine Phosphoproteome. J Proteome Res 14:3882-3891 (2015). Read more (PubMed: 26267517) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Question
    Answer

    ab51044
    -Note yet tested on frozen sections but has been tested on paraffin embedded sections
    fixation methods: formalin
    Recommended starting dilution: 1:50
    Positive control: testis, brain
    Negative control: protein is widely expressed so non-expressing tissue may be hard to find. Other controls are isotype control staining experiment, for example replacing ab51044 with Rabbit polyclonal IgG (ab27478)

    ab62435
    -Note yet tested on frozen sections
    fixation methods: 4% PFA tested in ICC only
    Recommended starting dilution:1:200
    Positive control: not yet tested in application, literature search may be helpful
    Negative control: not yet tested in application, literature search may be helpful, can also consider isotype control as noted above

    ab76469
    -Note yet tested on frozen sections
    fixation methods: 4% PFA tested in ICC only
    Recommended starting dilution: 1:100
    Positive control: not yet tested in application, literature search may be helpful
    Negative control: not yet tested in application, literature search may be helpful, can also consider isotype control as noted above

    ab75873
    -Note yet tested on frozen sections
    fixation methods: 4% PFA tested in ICC only
    Recommended starting dilution: 1:100
    Positive control: not yet tested in application, literature search may be helpful
    Negative control: not yet tested in application, literature search may be helpful, can also consider isotype control as noted above

    Since none of these 4 antibodies have yet been tested in IHC-Fr, we can not guarantee their utility in this application. However, we do have a testing discount promotion which you may be interested in. Briefly, if you buy any of these antibodies, test in IHC-Fr and provide a brief review of your procedures and results using our Abreview system (https://www.abcam.com/abreviews), this will activate a discount code I’ll provide which can then be used to get 1 free primary antibody per code. Please see below for a more detailed description of the promotion using ab51044 as an example.

    ==

    Thank you very much for your interest in ab51044.

    To our knowledge, ab/xxx/ has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab51044 now, test it in IHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab51044 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab51044 so please wait for my reply before ordering.

    2. Purchase ab51044 either by phone, fax, or online (www.abcam.com).

    3. Test it in IHC-Fr.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab51044 turns out to be unsuitable for IHC-Fr you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up