Overview

  • Product name

  • Description

    Rabbit polyclonal to ErbB 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Frog
  • Immunogen

    Synthetic peptide within Human ErbB 3 aa 650-750 (internal sequence). The exact sequence is proprietary.
    Database link: P21860

  • Positive control

    • IHC-P: Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Target

  • Function

    Binds and is activated by neuregulins and NTAK.
  • Tissue specificity

    Epithelial tissues and brain.
  • Involvement in disease

    Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Developmental stage

    Overexpressed in a subset of human mammary tumors.
  • Domain

    The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
  • Post-translational
    modifications

    Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • c erbB 3 antibody
    • c erbB3 antibody
    • Erb b2 receptor tyrosine kinase 3 antibody
    • ErbB 3 antibody
    • ERBB3 antibody
    • ERBB3 protein antibody
    • erbB3 S antibody
    • ERBB3_HUMAN antibody
    • Glial growth factor receptor antibody
    • HER 3 antibody
    • HER3 antibody
    • Human epidermal growth factor receptor 3 antibody
    • LCCS2 antibody
    • MDA BF 1 antibody
    • MGC88033 antibody
    • p180 ErbB3 antibody
    • p45 sErbB3 antibody
    • p85 sErbB3 antibody
    • proto-oncogene-like protein c ErbB 3 antibody
    • proto-oncogene-like protein c ErbB3 antibody
    • Proto-oncogene-like protein c-ErbB-3 antibody
    • Receptor tyrosine protein kinase erbB 3 antibody
    • Receptor tyrosine protein kinase erbB3 antibody
    • Receptor tyrosine-protein kinase erbB-3 antibody
    • Tyrosine kinase type cell surface receptor HER3 antibody
    • Tyrosine kinase-type cell surface receptor HER3 antibody
    • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for ErbB 3 using ab227775 at 1/100 dilution in immunohistochemical analysis.

References

ab227775 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal liver metastasis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Specification
Colorectal liver metastasis
Blocking step
TBS-0.01% Tween 20 + 10% normal horse serum + 1.5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 24 2018

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