Overview

  • Product name
  • Description
    Rabbit polyclonal to ErbB 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 41-56 of Human Erb B3.

  • Positive control
    • Mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab5470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).

Target

  • Function
    Binds and is activated by neuregulins and NTAK.
  • Tissue specificity
    Epithelial tissues and brain.
  • Involvement in disease
    Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Developmental stage
    Overexpressed in a subset of human mammary tumors.
  • Domain
    The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
  • Post-translational
    modifications
    Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c erbB 3 antibody
    • c erbB3 antibody
    • Erb b2 receptor tyrosine kinase 3 antibody
    • ErbB 3 antibody
    • ERBB3 antibody
    • ERBB3 protein antibody
    • erbB3 S antibody
    • ERBB3_HUMAN antibody
    • Glial growth factor receptor antibody
    • HER 3 antibody
    • HER3 antibody
    • Human epidermal growth factor receptor 3 antibody
    • LCCS2 antibody
    • MDA BF 1 antibody
    • MGC88033 antibody
    • p180 ErbB3 antibody
    • p45 sErbB3 antibody
    • p85 sErbB3 antibody
    • proto-oncogene-like protein c ErbB 3 antibody
    • proto-oncogene-like protein c ErbB3 antibody
    • Proto-oncogene-like protein c-ErbB-3 antibody
    • Receptor tyrosine protein kinase erbB 3 antibody
    • Receptor tyrosine protein kinase erbB3 antibody
    • Receptor tyrosine-protein kinase erbB-3 antibody
    • Tyrosine kinase type cell surface receptor HER3 antibody
    • Tyrosine kinase-type cell surface receptor HER3 antibody
    • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
    see all

Images

  • Anti-ErbB 3 antibody (ab5470) + Mouse brain lysate

    Predicted band size: 148 kDa
    Observed band size: 155 kDa
    why is the actual band size different from the predicted?



    Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

    HC = hepatocarcinoma.

References

This product has been referenced in:
  • Xu J  et al. HER2 overexpression reverses the relative resistance of EGFR-mutant H1975 cell line to gefitinib. Oncol Lett 12:5363-5369 (2016). WB ; Human . Read more (PubMed: 28105244) »
  • Wan J  et al. Modification of HER2 pre-mRNA alternative splicing and its effects on breast cancer cells. Int J Cancer 124:772-7 (2009). WB ; Human . Read more (PubMed: 19035464) »
See all 3 Publications for this product

Customer reviews and Q&As

Question
Answer

DISCOUNT CODE: XXXXXXXX Expiration date: 16-01-2012 I am very pleased to hear you would like to accept our offer and test ab5470 in Flow Cytometry. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. PS: The positive control cell line we can recommend is Jurkat cell line, COLO205 cells or IFN alpha stimulated KAS-6/1, ANBL-6 cell lines. The information about positive control cell lines is easily available in literature so I would recommend searching PubMed.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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