Recombinant Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E186] to ErbB3 / HER3 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free
See all ErbB3 / HER3 primary antibodies -
Description
Rabbit monoclonal [E186] to ErbB3 / HER3 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody recognises ErbB3/HER3. WB samples: should avoid boiling.
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Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC or IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF-7 cell lysate.
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General notes
ab189333 is the carrier-free version of ab32121.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E186 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 185 kDa (predicted molecular weight: 149 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 185 kDa (predicted molecular weight: 149 kDa). |
Target
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Function
Binds and is activated by neuregulins and NTAK. -
Tissue specificity
Epithelial tissues and brain. -
Involvement in disease
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Developmental stage
Overexpressed in a subset of human mammary tumors. -
Domain
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
Post-translational
modificationsLigand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2065 Human
- Entrez Gene: 29496 Rat
- Omim: 190151 Human
- SwissProt: P21860 Human
- SwissProt: Q62799 Rat
- Unigene: 118681 Human
- Unigene: 10228 Rat
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Alternative names
- c erbB 3 antibody
- c erbB3 antibody
- Erb b2 receptor tyrosine kinase 3 antibody
see all
Images
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This data was developed using ab32121, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ERBB3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: MCF7 whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab32121 observed at 185 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab32121 was shown to specifically recognize ERBB3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when knock out samples were examined. Wild-type and ERBB3 knockout samples were subjected to SDS-PAGE. ab32121 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/1000 dilution + MDA-MB-435S (human ductal carcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDaThis data was developed using ab32121, the same antibody clone in a different buffer formulation.
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All lanes : Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 2.685 µg/ml (purified)
Lane 1 : MDA-MB-435S (human mammary gland ductal carcinoma melanocyte) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 149 kDa
Exposure time: 37 secondsThis data was developed using ab32121, the same antibody clone in a different buffer formulation.
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Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/500 dilution (unpurified) + MCF-7 cell lysate
Predicted band size: 149 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?This data was developed using ab32121, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab189333 has not yet been referenced specifically in any publications.