Overview

  • Product name

    Anti-ErbB 3 antibody [SP71]
    See all ErbB 3 primary antibodies
  • Description

    Rabbit monoclonal [SP71] to ErbB 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ErbB 3 aa 650-750 (internal sequence). The exact sequence is proprietary.
    Database link: P21860

  • Positive control

    • FC: MCF7 cells IHC: human small intestine and human colon tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab93739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

1/100 for 30 mins at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function

    Binds and is activated by neuregulins and NTAK.
  • Tissue specificity

    Epithelial tissues and brain.
  • Involvement in disease

    Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Developmental stage

    Overexpressed in a subset of human mammary tumors.
  • Domain

    The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
  • Post-translational
    modifications

    Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • c erbB 3 antibody
    • c erbB3 antibody
    • Erb b2 receptor tyrosine kinase 3 antibody
    • ErbB 3 antibody
    • ERBB3 antibody
    • ERBB3 protein antibody
    • erbB3 S antibody
    • ERBB3_HUMAN antibody
    • Glial growth factor receptor antibody
    • HER 3 antibody
    • HER3 antibody
    • Human epidermal growth factor receptor 3 antibody
    • LCCS2 antibody
    • MDA BF 1 antibody
    • MGC88033 antibody
    • p180 ErbB3 antibody
    • p45 sErbB3 antibody
    • p85 sErbB3 antibody
    • proto-oncogene-like protein c ErbB 3 antibody
    • proto-oncogene-like protein c ErbB3 antibody
    • Proto-oncogene-like protein c-ErbB-3 antibody
    • Receptor tyrosine protein kinase erbB 3 antibody
    • Receptor tyrosine protein kinase erbB3 antibody
    • Receptor tyrosine-protein kinase erbB-3 antibody
    • Tyrosine kinase type cell surface receptor HER3 antibody
    • Tyrosine kinase-type cell surface receptor HER3 antibody
    • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody
    • v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling ErbB 3 with ab93739 at 1/100 dilution (5.5 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on human colon, performed on a Leica Biosystems BOND™ RX instrument
    The section was incubated with ab93793 for 30 mins at room temperature

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human small intestine tissue sections labeling ErbB 3 with ab93739 at 1/100 dilution (5.5 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on human small intestine, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab93793 for 30 mins at room temperature.

  • Flow cytometry analysis of MCF7 (human breast adenocarcinoma epithelial cell) labeling ErbB 3 with purified ab93739 at 1/50 dilution (10.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

  • An immunohistochemical staining of human small intestine labelling c-ErB-3 / HER-3 (SP71) with ab93739 at dilution 1:100.

  • ab93739 at 1/100 dilution staining ErbB 3 in formalin-fixed, paraffin-embedded Human small intestine tissue.

References

This product has been referenced in:

  • Ma S  et al. ErbB3 Ligand Heregulin1 Is a Major Mitogenic Factor for Uncontrolled Lung Cancer Cell Proliferation. Neoplasia 21:343-352 (2019). Read more (PubMed: 30831376) »
  • Hayes DA  et al. ERBB3: A potential serum biomarker for early detection and therapeutic target for devil facial tumour 1 (DFT1). PLoS One 12:e0177919 (2017). IHC-P . Read more (PubMed: 28591206) »
See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal liver metastasis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 8
Permeabilization
No
Specification
Colorectal liver metastasis
Blocking step
TBS-0.01% Tween 20 + 10% normal horse serum + 1.5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 24 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up