Overview

  • Product name

    Anti-ErbB 3 (phospho Y1222) antibody [EPR5807]
    See all ErbB 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5807] to ErbB 3 (phospho Y1222)
  • Host species

    Rabbit
  • Specificity

    ab133445 only detects ErbB 3 phosphorylated at Tyrosine 1222.
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to a region surrounding Tyrosine 1222 of Human ErbB 3.

  • Positive control

    • Lysate of SKBR3 cells treated with neuregulin.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa).
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Binds and is activated by neuregulins and NTAK.
    • Tissue specificity

      Epithelial tissues and brain.
    • Involvement in disease

      Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • Developmental stage

      Overexpressed in a subset of human mammary tumors.
    • Domain

      The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
    • Post-translational
      modifications

      Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • c erbB 3 antibody
      • c erbB3 antibody
      • Erb b2 receptor tyrosine kinase 3 antibody
      • ErbB 3 antibody
      • ERBB3 antibody
      • ERBB3 protein antibody
      • erbB3 S antibody
      • ERBB3_HUMAN antibody
      • Glial growth factor receptor antibody
      • HER 3 antibody
      • HER3 antibody
      • Human epidermal growth factor receptor 3 antibody
      • LCCS2 antibody
      • MDA BF 1 antibody
      • MGC88033 antibody
      • p180 ErbB3 antibody
      • p45 sErbB3 antibody
      • p85 sErbB3 antibody
      • proto-oncogene-like protein c ErbB 3 antibody
      • proto-oncogene-like protein c ErbB3 antibody
      • Proto-oncogene-like protein c-ErbB-3 antibody
      • Receptor tyrosine protein kinase erbB 3 antibody
      • Receptor tyrosine protein kinase erbB3 antibody
      • Receptor tyrosine-protein kinase erbB-3 antibody
      • Tyrosine kinase type cell surface receptor HER3 antibody
      • Tyrosine kinase-type cell surface receptor HER3 antibody
      • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
      see all

    Images

    • All lanes : Anti-ErbB 3 (phospho Y1222) antibody [EPR5807] (ab133445) at 1/1000 dilution

      Lane 1 : SKBR3 cell lysate
      Lane 2 : Lysate of SKBR3 cells treated with neuregulin.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 148 kDa
      Observed band size: 185 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Jacobi N  et al. Organotypic three-dimensional cancer cell cultures mirror drug responsesin vivo: lessons learned from the inhibition of EGFR signaling. Oncotarget 8:107423-107440 (2017). Read more (PubMed: 29296175) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Breast cancer cells)
    Permeabilization
    Yes - 0.5% Triton-x
    Specification
    Breast cancer cells
    Blocking step
    5% FBS, 1% BSA, 0.05% Tween /PBS as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jan 15 2018

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Normal tissue types and breast tumors)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH8.8
    Permeabilization
    No
    Specification
    Normal tissue types and breast tumors
    Blocking step
    MACH1 Sniper buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Formaldehyde

    Mr. Malcolm Lim

    Verified customer

    Submitted Nov 30 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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