Overview

  • Product name

    Anti-ErbB 3 (phospho Y1289) antibody [EPNCIR124]
    See all ErbB 3 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR124] to ErbB 3 (phospho Y1289)
  • Host species

    Rabbit
  • Specificity

    ab133433 only detects ErbB3 phosphorylated at Tyrosine 1289.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide corresponding to a region surrounding Tyrosine 1289 of Mouse ErbB3.

  • Positive control

    • Lysate of MCF7 cells treated with neuregulin.
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institutes Center for Cancer Research and the lab of Terry Van Dyke. View antibodies from NCI Center for Cancer Research Collaboration.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Binds and is activated by neuregulins and NTAK.
    • Tissue specificity

      Epithelial tissues and brain.
    • Involvement in disease

      Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • Developmental stage

      Overexpressed in a subset of human mammary tumors.
    • Domain

      The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
    • Post-translational
      modifications

      Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • c erbB 3 antibody
      • c erbB3 antibody
      • Erb b2 receptor tyrosine kinase 3 antibody
      • ErbB 3 antibody
      • ERBB3 antibody
      • ERBB3 protein antibody
      • erbB3 S antibody
      • ERBB3_HUMAN antibody
      • Glial growth factor receptor antibody
      • HER 3 antibody
      • HER3 antibody
      • Human epidermal growth factor receptor 3 antibody
      • LCCS2 antibody
      • MDA BF 1 antibody
      • MGC88033 antibody
      • p180 ErbB3 antibody
      • p45 sErbB3 antibody
      • p85 sErbB3 antibody
      • proto-oncogene-like protein c ErbB 3 antibody
      • proto-oncogene-like protein c ErbB3 antibody
      • Proto-oncogene-like protein c-ErbB-3 antibody
      • Receptor tyrosine protein kinase erbB 3 antibody
      • Receptor tyrosine protein kinase erbB3 antibody
      • Receptor tyrosine-protein kinase erbB-3 antibody
      • Tyrosine kinase type cell surface receptor HER3 antibody
      • Tyrosine kinase-type cell surface receptor HER3 antibody
      • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
      see all

    Images

    • All lanes : Anti-ErbB 3 (phospho Y1289) antibody [EPNCIR124] (ab133443) at 1/10000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : MCF7 cell lysate treated with neuregulin

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/10000 dilution

      Predicted band size: 148 kDa
      Observed band size: 185 kDa
      why is the actual band size different from the predicted?

    References

    ab133443 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Melanoma)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    15 µg
    Specification
    Melanoma
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Apr 09 2019

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