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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-ErbB2 / HER2 Affibody® Molecule (Biotin) (ab31890)

  • Datasheet
Q&A (4)References (3)

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Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 Affibody® Molecule (Biotin) (ab31890)

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    Overview

    • Product name

      Anti-ErbB2 / HER2 Affibody® Molecule (Biotin)
      See all ErbB2 / HER2 affibody® molecule
    • Conjugation

      Biotin
    • Conjugation notes

      This molecule is biotinylated at the unique C-terminal cysteine using a maleimide-biotin reagent.
    • Tested applications

      Suitable for: IHC-Fr, ICC/IF, Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant full length protein corresponding to ErbB2/ HER2.

    • General notes

      This product is a recombinant protein produced in E.coli.

      What are Affibody Molecules?
      Affibody® affinity ligands are unique research reagents, produced using innovative protein-engineering technologies. They are small, simple proteins composed of a three-helix bundle based on the scaffold of one of the IgG-binding domains of Protein A. Protein A is a surface protein from the bacterium Staphylococcus aureus. This scaffold has excellent features as an affinity ligand and can be designed to bind with high affinity to any given target protein. The domain consists of 58 amino acids, 13 of which are randomized to generate Affibody® libraries with a large number of ligand variants. Thus, the libraries consist of a multitude of protein ligands with an identical backbone and variable surface-binding properties. In function, Affibody® Molecules mimic monoclonal antibodies. Compared to antibodies, the most striking dissimilarity of Affibody® Molecules is the small size. Affibody® Molecules have a molecular weight of 6kDa, compared to the molecular weight of antibodies, which is 150kDa. In spite of its small size, the binding site of Affibody® Molecules is similar to that of an antibody. The advantages of Affibody® Molcules over antibodies are: -their small size -the simple structure of the molecules -its robust physical properties; able to withstand a broad range of analytical conditions, including extreme pH and elevated temperature -its ability to fold correctly intracellularly -the fast and cost effective production in bacteria -the potential to couple Affibody® Molecules in multimeric constructs Affibody® Molecules have highly competitive properties for applications within affinity purification, sample preparation, protein detection and in vitro diagnostics.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Store In the Dark.
    • Storage buffer

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 0.328% Sodium phosphate, 0.87% Sodium chloride
    • Concentration information loading...
    • Purification notes

      The purity of this product is >98% as determined by RP-HPLC analysis.
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Receptor Tyrosine Kinases
      • Signal Transduction
      • Growth Factors/Hormones
      • EGF
      • Cancer
      • Growth factors
      • EGF
      • Cancer
      • Tumor immunology
      • Tumor-associated antigens
      • Cancer
      • Tumor biomarkers
      • Oncoproteins
    • Function

      Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
      In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
    • Tissue specificity

      Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
    • Involvement in disease

      Hereditary diffuse gastric cancer
      Glioma
      Ovarian cancer
      Lung cancer
      Gastric cancer
      Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
    • Cellular localization

      Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
    • Target information above from: UniProt accession P04626 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
      • C erb B2/neu protein
      • CD340
      • CD340 antigen
      • Cerb B2/neu protein
      • CerbB2
      • Erb b2 receptor tyrosine kinase 2
      • ErbB-2 proto-oncogene
      • ERBB2
      • ERBB2_HUMAN
      • HER 2
      • HER 2/NEU
      • HER2
      • Herstatin
      • Human epidermal growth factor receptor 2
      • Metastatic lymph node gene 19 protein
      • MLN 19
      • MLN19
      • NEU
      • NEU proto oncogene
      • Neuro/glioblastoma derived oncogene homolog
      • Neuroblastoma/glioblastoma derived oncogene homolog
      • NGL
      • p185erbB2
      • Proto-oncogene c-ErbB-2
      • Proto-oncogene Neu
      • Receptor tyrosine-protein kinase erbB-2
      • TKR1
      • Tyrosine kinase type cell surface receptor HER2
      • Tyrosine kinase-type cell surface receptor HER2
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2
      • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
      • V erb b2 avian erythroblastic leukemia viral oncoprotein 2
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
      • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
      • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
      see all
    • Database links

      • Entrez Gene: 2064 Human
      • Omim: 164870 Human
      • SwissProt: P04626 Human
      • Unigene: 446352 Human

      Associated products

      • Recombinant Protein

        • Recombinant human ErbB2 / HER2 protein (ab168896)

      Applications

      The Abpromise guarantee

      Our Abpromise guarantee covers the use of ab31890 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Abreviews Notes
      IHC-Fr
      Use at an assay dependent dilution.
      Staining of paraffin embedded tissues is not recommended.
      AFFIBODY® MOLECULES ARE PROTOCOL SPECIFIC. PLEASE REFER TO THE "PROTOCOLS" SECTION.
      ICC/IF
      Use at an assay dependent dilution.
      Flow Cyt
      Use at an assay dependent dilution.
      Notes
      IHC-Fr
      Use at an assay dependent dilution.

      Staining of paraffin embedded tissues is not recommended.
      AFFIBODY® MOLECULES ARE PROTOCOL SPECIFIC. PLEASE REFER TO THE "PROTOCOLS" SECTION.
      ICC/IF
      Use at an assay dependent dilution.
      Flow Cyt
      Use at an assay dependent dilution.

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 Affibody® Molecule (Biotin) (ab31890)
        Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 Affibody® Molecule (Biotin) (ab31890)

        Cells of the human mammary gland cell line SK-BR3 were stained with biotin conjugated Anti-ErbB 2 Affibody® molecule (ab31890). The staining was localized to the membrane of the ErbB2 / HER2 expressing human mammary gland cells. Nuclei were counter stained with DAPI (blue fluorescence).

         

        The SK-BR3 cells were stained for 30 minutes at a concentration of 1-5ug Affibody® molecule/ml.

      Datasheets and documents

      • Datasheet download

        Download

      Protocols

      • Fluorescence Staining Of Cells And Tissues
      • Fluorescence Staining of Cells for Flow Cytometry

      Click here to view the general protocols

      References (3)

      Publishing research using ab31890? Please let us know so that we can cite the reference in this datasheet.

      ab31890 has been referenced in 3 publications.

      • Lim H & Moon D Microscopic and nanoscopic protein imaging by SIMS and helium ion microscopy. Biointerphases 15:038501 (2020). PubMed: 32590901
      • Grindel BJ  et al. Mammalian Expression and In Situ Biotinylation of Extracellular Protein Targets for Directed Evolution. ACS Omega 5:25440-25455 (2020). PubMed: 33043224
      • Liu X  et al. Affinity-Tuned ErbB2 or EGFR Chimeric Antigen Receptor T Cells Exhibit an Increased Therapeutic Index against Tumors in Mice. Cancer Res 75:3596-607 (2015). PubMed: 26330166

      Customer reviews and Q&As

      Q&A
      Submit a question

      1-4 of 4 Q&A

      Question

      Thank you for your answer. I would like to receive , if possible, an offer with 5 % of discount for ab40048.
      with my best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Oct 29 2012

      Answer

      Thank you for contacting us.

      Please use the code 5PC-OFF-T7T62 to get 5% discount when ordering the protein online.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Oct 29 2012

      Question

      Thank you for your answer and suggestions. I decided to order protein ab40048 and I would like to know if it possible to have a test sample or a discount for test the protein.

      With my best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Oct 29 2012

      Answer

      Thank you for your email.

      Unfortunately we do not provide free of charge samples. However in order to help our customers we provide Abtrial programme (https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount). The abtrial is possible in case of ab40048 only if you are interested in using this protein in applications other than ELISA, Inhibition assay, SDS-PAGE and WB.

      The Abtrial will provide you your next product free of charge if you buy and test ab40048.

      Please let me know how you would like to proceed.

      Read More

      Abcam Scientific Support

      Answered on Oct 29 2012

      Question

      I have ordered ab31890 biotin-affibody. Could you suggest me an erbb2 protein compatible with this affibody?

      With my best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Oct 18 2012

      Answer

      Thank you for contacting us.

      We have ErbB2 proteins available however we haven't tried these with ab31890. I am sorry we will not be able to suggest the compatible product however technically the affibody should work with these proteins.

      The catalogue numbers are;

      ab40048

      ab60866

      ab63288

      ab85602
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Oct 18 2012

      Question

      I wish to use this molecule to probe for my ErbB2 mutant, and am wondering if the epitope of the affibody would be within 100 amino acids of the missing region. The missing region is aa 634-649.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 08 2006

      Answer

      Thank you for your enquiry. We have not yet mapped the binding site of the Anti-HER2 Affibody molecule on the HER2 receptor. However, it was raised against the whole extracellular part of HER2 and may therefore bind to any of the four extracellular domains. We do know that the Affibody binding epitope is distinct from Herceptin's (Trastuzumab) since Trastuzumab and Anti-HER2 Affibody molecule do not block each other's binding. I am sorry that we couldn't be more specific at this time. We will probably do some mapping in the future.

      Read More

      Abcam Scientific Support

      Answered on Jun 12 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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