Recombinant Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1045Y] to ErbB2 / HER2
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-ErbB2 / HER2 antibody [EP1045Y]
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit monoclonal [EP1045Y] to ErbB2 / HER2 -
Host species
Rabbit -
Specificity
ab134182 detects ErbB 2 phosphorylated at Tyr1248 as well as unphosphorylated ErbB 2. -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
within Human ErbB2/ HER2 aa 1200 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P04626 -
Positive control
- WB: HeLa, SKBR-3 cell lysate; IHC-P: Human breast carcinoma tissue; ICC/IF: SKBR cells; IP: HeLa.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 3.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1045Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab134182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 137 kDa. | |
| IP | 1/30. For unpurifed use at 1/50. |
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| ICC/IF | 1/250 - 1/500. | |
| IHC-P | 1/1600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/100 -1/250. |
Target
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Function
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
Tissue specificity
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
Involvement in disease
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
Cellular localization
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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Database links
- Entrez Gene: 2064 Human
- Omim: 164870 Human
- SwissProt: P04626 Human
- Unigene: 446352 Human
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Alternative names
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
Images
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![Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)](https://www.abcam.com/ps/products/134/ab134182/Images/ab134182-330945-anti-erbb-2-antibody-ep1045y-western-blot.jpg)
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/1000 dilution + 4T1(Mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 137 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)](https://www.abcam.com/ps/products/134/ab134182/Images/ab134182-300477-anti-erbb-2-antibody-ep1045y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling EErbB2 / HER2 with Purified ab134182 at 1:1600 dilution (0.68 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)](https://www.abcam.com/ps/products/134/ab134182/Images/ab134182-300530-anti-erbb-2-antibody-ep1045y-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)ab134182 (purified) at 1:30 dilution (2µg) immunoprecipitating ErbB2 / HER2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab134182 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134182 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)](https://www.abcam.com/ps/products/134/ab134182/Images/ab134182-300182-anti-erbb-2-antibody-ep1045y-western-blot.jpg)
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/10000 dilution (purified) + SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 137 kDa
Blocking and diluting buffer : 5% NFDM/TBST -
![Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)](https://www.abcam.com/ps/products/134/ab134182/Images/ab134182-300181-anti-erbb-2-antibody-ep1045y-western-blot.jpg)
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/1000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 137 kDa
Blocking and diluting buffer : 5% NFDM/TBST -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)](https://www.abcam.com/ps/products/134/ab134182/Images/ab134182-148504-anti-erbb-2-antibody-ep1045y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ErbB2 / HER2 with ab134182 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9.0).
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![Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)](https://www.abcam.com/ps/products/134/ab134182/Images/ab134182-148503-anti-erbb-2-antibody-ep1045y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)Immunofluorescent analysis of SKBR cells labelling ErbB2 / HER2 with ab134182 at 1/250 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)](https://www.abcam.com/ps/products/134/ab134182/Images/ab134182-265170-anti-erbb-2-antibody-ep1045y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)Immunocytochemistry/Immunofluorescence analysis of SK-BR-3 (human mammary gland adenocarcinoma) labelling ErbB2 / HER2 with purified ab134182 at 1/125. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)](https://www.abcam.com/ps/products/134/ab134182/Images/ab134182-148505-anti-erbb-2-antibody-ep1045y-western-blot.jpg)
Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/10000 dilution + SKBR-3 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 137 kDa -
![Other - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)](https://www.abcam.com/ps/products/134/ab134182/Images/ab134182-148502-anti-erbb-2-antibody-ep1045y.jpg)
Other - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
Protocols
References (28)
ab134182 has been referenced in 28 publications.
- Cho KF et al. Chaperone mediated detection of small molecule target binding in cells. Nat Commun 11:465 (2020). PubMed: 31974362
- Wang Y et al. GEFT protein expression in digestive tract malignant tumors and its clinical significance. Oncol Lett 18:5577-5590 (2019). PubMed: 31620201
- Kobayashi Y et al. Clozapine-dependent inhibition of EGF/neuregulin receptor (ErbB) kinases. Transl Psychiatry 9:181 (2019). PubMed: 31371697
- Huang F et al. Simultaneous Inhibition of EGFR and HER2 via Afatinib Augments the Radiosensitivity of Nasopharyngeal Carcinoma Cells. J Cancer 10:2063-2073 (2019). PubMed: 31205567
- Cao H et al. Lymphoepithelioma-like gastric carcinoma treated with partial gastrectomy: Two case reports. Oncol Lett 18:545-552 (2019). PubMed: 31289526
