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Tags & Cell Markers Cell Type Markers Tumor Associated
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RecombinantRabMAb

Recombinant Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (32)

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Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
  • Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
  • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
  • OI-RD Scanning - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
  • Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1045Y] to ErbB2 / HER2
  • Suitable for: WB, IP, ICC/IF, IHC-P
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-ErbB2 / HER2 antibody [EP1045Y]
    See all ErbB2 / HER2 primary antibodies
  • Description

    Rabbit monoclonal [EP1045Y] to ErbB2 / HER2
  • Host species

    Rabbit
  • Specificity

    ab134182 detects ErbB 2 phosphorylated at Tyr1248 as well as unphosphorylated ErbB 2.
  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, SKBR-3 cell lysate; IHC-P: Human breast carcinoma tissue; ICC/IF: SKBR cells; IP: HeLa.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 3.00 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1045Y
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cancer
    • Growth factors
    • EGF
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Cancer
    • Tumor biomarkers
    • Oncoproteins

Associated products

  • Alternative Versions

    • Anti-ErbB2 / HER2 antibody [EP1045Y] - BSA and Azide free (ab194979)
    • Alexa Fluor® 488 Anti-ErbB2 / HER2 antibody [EP1045Y] (ab275994)
    • Alexa Fluor® 647 Anti-ErbB2 / HER2 antibody [EP1045Y] (ab281578)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 137 kDa.
IP
1/30.

For unpurifed use at 1/50.

ICC/IF (2)
1/250 - 1/500.
IHC-P (1)
1/1600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/100 -1/250. 

Notes
WB
1/1000. Predicted molecular weight: 137 kDa.
IP
1/30.

For unpurifed use at 1/50.

ICC/IF
1/250 - 1/500.
IHC-P
1/1600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/100 -1/250. 

  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
      In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
    • Tissue specificity

      Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
    • Involvement in disease

      Hereditary diffuse gastric cancer
      Glioma
      Ovarian cancer
      Lung cancer
      Gastric cancer
      Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
    • Cellular localization

      Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.
    • Target information above from: UniProt accession P04626 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2064 Human
      • Entrez Gene: 13866 Mouse
      • Omim: 164870 Human
      • SwissProt: P04626 Human
      • SwissProt: P70424 Mouse
      • Unigene: 446352 Human
      • Unigene: 290822 Mouse
      • Alternative names

        • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
        • C erb B2/neu protein antibody
        • CD340 antibody
        • CD340 antigen antibody
        • Cerb B2/neu protein antibody
        • CerbB2 antibody
        • Erb b2 receptor tyrosine kinase 2 antibody
        • ErbB-2 proto-oncogene antibody
        • ERBB2 antibody
        • ERBB2_HUMAN antibody
        • HER 2 antibody
        • HER 2/NEU antibody
        • HER2 antibody
        • Herstatin antibody
        • Human epidermal growth factor receptor 2 antibody
        • Metastatic lymph node gene 19 protein antibody
        • MLN 19 antibody
        • MLN19 antibody
        • NEU antibody
        • NEU proto oncogene antibody
        • Neuro/glioblastoma derived oncogene homolog antibody
        • Neuroblastoma/glioblastoma derived oncogene homolog antibody
        • NGL antibody
        • p185erbB2 antibody
        • Proto-oncogene c-ErbB-2 antibody
        • Proto-oncogene Neu antibody
        • Receptor tyrosine-protein kinase erbB-2 antibody
        • TKR1 antibody
        • Tyrosine kinase type cell surface receptor HER2 antibody
        • Tyrosine kinase-type cell surface receptor HER2 antibody
        • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody
        • V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody
        • V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody
        • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
        • V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
        • Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
        see all

      Images

      • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/1000 dilution + 4T1(Mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 137 kDa
        Observed band size: 180 kDa why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling EErbB2 / HER2 with Purified ab134182 at 1:1600 dilution (0.68 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      • Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Immunoprecipitation - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

        ab134182 (purified) at 1:30 dilution (2µg) immunoprecipitating ErbB2 / HER2 in HeLa whole cell lysate.
        Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
        Lane 2 (+): ab134182 & HeLa whole cell lysate
        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134182 in HeLa whole cell lysate
        For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
        Blocking and diluting buffer: 5% NFDM/TBST.

      • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/10000 dilution (purified) + SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 137 kDa



        Blocking and diluting buffer : 5% NFDM/TBST
      • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/1000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 137 kDa



        Blocking and diluting buffer : 5% NFDM/TBST
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

        Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ErbB2 / HER2 with ab134182 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9.0).

      • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

        Immunofluorescent analysis of SKBR cells labelling ErbB2 / HER2 with ab134182 at 1/250 dilution.

      • Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

        Immunocytochemistry/Immunofluorescence analysis of SK-BR-3 (human mammary gland adenocarcinoma) labelling ErbB2 / HER2 with purified ab134182 at 1/125. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

        Control: PBS only

      • Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Western blot - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182) at 1/10000 dilution + SKBR-3 cell lysate at 10 µg

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 137 kDa

      • OI-RD Scanning - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        OI-RD Scanning - Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)
        Anti-ErbB2 / HER2 antibody [EP1045Y] (ab134182)

      Protocols

      • Flow cytometry protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (32)

      Publishing research using ab134182? Please let us know so that we can cite the reference in this datasheet.

      ab134182 has been referenced in 32 publications.

      • Cho KF  et al. Chaperone mediated detection of small molecule target binding in cells. Nat Commun 11:465 (2020). PubMed: 31974362
      • Huang Z  et al. Clinicopathological value of ErbB2 gene and protein expression in osteochondroma. Acta Orthop Traumatol Turc 54:34-41 (2020). PubMed: 32175895
      • Cao Y  et al. Mining the Prognostic Value of HNRNPAB and Its Function in Breast Carcinoma. Int J Genomics 2020:3750673 (2020). PubMed: 32509843
      • Azimi T  et al. Cancer cells grown in 3D under fluid flow exhibit an aggressive phenotype and reduced responsiveness to the anti-cancer treatment doxorubicin. Sci Rep 10:12020 (2020). PubMed: 32694700
      • Wang Y  et al. GEFT protein expression in digestive tract malignant tumors and its clinical significance. Oncol Lett 18:5577-5590 (2019). PubMed: 31620201
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review

      Filter by Application

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      1-3 of 3 Abreviews

      Immunocytochemistry/ Immunofluorescence abreview for Anti-ErbB 2 antibody [EP1045Y]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Dog Cell (Breast tissue)
      Permeabilization
      Yes - PBS 1X+Triton 0.3%
      Specification
      Breast tissue
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
      Fixative
      Paraformaldehyde
      Read More

      Msr. Emmanuel Bouchaert

      Verified customer

      Submitted Feb 12 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ErbB 2 antibody [EP1045Y]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Dog Tissue sections (Breast tissue)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: CC1 buffer (high pH Tris based)
      Permeabilization
      No
      Specification
      Breast tissue
      Fixative
      formaline 10%
      Read More

      Msr. Emmanuel Bouchaert

      Verified customer

      Submitted Jan 24 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-ErbB 2 antibody [EP1045Y]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Specification
      HeLa
      Permeabilization
      No
      Fixative
      Paraformaldehyde
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      Submitted Mar 27 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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