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I am going to use this Anti-ErbB2 Affibody® Molecule in FACS to determine the presence of ErbB2 on the cell surface. Would you please tell me which control molecule I should use as the negative control?
Asked on Aug 21 2012
Thank you for contacting us.
We do not have a FITC-conjugated control for Affibodies®. One palternative would be to conjugate a similar Affibody® to FITC, such as ab31908, which is directed against TNF alpha. The two Affibodies® are similar in structure (head-to-tail dimers) but the suitablility of using an anti-TNF alpha Affibody® as a negative control depends on knowing your samples do not contain TNF alpha.
The online datasheet for the ab31908 is here:
Click here (or use the following: https://www.abcam.com/index.html?datasheet=31908).
The datasheet for our FITC conjugation kit is here:
Click here (or use the following: https://www.abcam.com/index.html?datasheet=102885).
Please do not hesitate to contact us if you need any more advice or information.
Answered on Aug 21 2012