Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5808(2)] to ErbB3 / HER3 (phospho Y1276) - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Product nameAnti-ErbB3 / HER3 (phospho Y1276) antibody [EPR5808(2)] - BSA and Azide free
DescriptionRabbit monoclonal [EPR5808(2)] to ErbB3 / HER3 (phospho Y1276) - BSA and Azide free
Specificityab232058 detects ErbB 3 only when phosphorylated at tyrosine 1276.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ErbB3/ HER3 (phospho Y1276). The exact sequence is proprietary.
- WB: Neuregulin treated T-47D cell lysate.
ab232058 is the carrier-free version of ab134900. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232058 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab232058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa).|
All lanes : Anti-ErbB3 / HER3 (phospho Y1276) antibody [EPR5808(2)] (ab134900) at 1/10000 dilution
Lane 1 : Un-treated T-47D (Human ductal breast epithelial tumor cell line) cell lysate
Lane 2 : Neuregulin-treated T-47D (Human ductal breast epithelial tumor cell line) cell lysate
All lanes : HRP-labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 148 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134900).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232058 has not yet been referenced specifically in any publications.