Key features and details
- Rabbit polyclonal to ErbB3 / HER3 (phospho Y1328)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ErbB3 / HER3 (phospho Y1328) antibody
See all ErbB3 / HER3 primary antibodies
DescriptionRabbit polyclonal to ErbB3 / HER3 (phospho Y1328)
ab131444 detects endogenous levels of ErbB3 / HER3 only when phosphorylated at Tyrosine 1328.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- A431 cell extract treated with EGF; MCF7 cells; Human breast carcinoma tissue.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131444 was purified by using epitope specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab131444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionBinds and is activated by neuregulins and NTAK.
Tissue specificityEpithelial tissues and brain.
Involvement in diseaseDefects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
Developmental stageOverexpressed in a subset of human mammary tumors.
DomainThe cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
modificationsLigand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- c erbB 3 antibody
- c erbB3 antibody
- Erb b2 receptor tyrosine kinase 3 antibody
All lanes : Anti-ErbB3 / HER3 (phospho Y1328) antibody (ab131444) at 1/500 dilution
Lane 1 : A431 cell extract, untreated
Lane 2 : A431 cell extract, treated with EGF
Predicted band size: 148 kDa
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling ErbB3 / HER3 (phospho Y1328) with ab131444 antibody at 1/50 dilution. The image on the right is treated with the synthesized peptide.
Immunofluorescence of methanol-fixed MCF7 cells labelling EErbB3 / HER3 (phospho Y1328) with ab131444 antibody at 1/100 dilution.
ab131444 has not yet been referenced specifically in any publications.