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Immunocytochemistry/ Immunofluorescence abreview for Anti-ErbB 4 (phospho Y1284) antibody

Excellent
Abreviews
Go to datasheet (ab61059)
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA and Normal Goat Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Sample
Rat Cell (Heart sections)
Specification
Heart sections
Permeabilization
Yes - Tween20 0.05%
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 5% and Tween20 0.05% in TBS
Dilution
1/30

Secondary antibody

Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor­ 488)
Dilution
1/200
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Simon Pascual

Verified customer

Submitted Apr 15 2014