Overview

  • Product name
  • Description
    Rabbit polyclonal to Erbin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Erbin (N terminal).
    Sequence:

    RELDVSKNGIQEFPENIKNC

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 157 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state.
  • Tissue specificity
    Highly expressed in brain, heart, kidney, muscle and stomach, followed by liver, spleen and intestine.
  • Sequence similarities
    Belongs to the LAP (LRR and PDZ) protein family.
    Contains 17 LRR (leucine-rich) repeats.
    Contains 1 PDZ (DHR) domain.
  • Cellular localization
    Cell junction > hemidesmosome. Found in hemidesmosomes, which are cell-substrate adhesion complexes in stratified epithelia. In transfected cells, either diffusely distributed over the cytoplasm or concentrated at the basolateral membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Densin-180-like protein antibody
    • Erbb2-interacting protein antibody
    • Erbb2ip antibody
    • Erbin antibody
    • LAP2 antibody
    • LAP2_HUMAN antibody
    • Protein LAP2 antibody
    see all

Images

  • All lanes : Anti-Erbin antibody (ab55930) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 157 kDa
    Observed band size: 172 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Liu H  et al. LET-413/Erbin acts as a RAB-5 effector to promote RAB-10 activation during endocytic recycling. J Cell Biol 217:299-314 (2018). Read more (PubMed: 29079669) »
  • Pan P & Dobrowsky RT Differential expression of neuregulin-1 isoforms and downregulation of erbin are associated with Erb B2 receptor activation in diabetic peripheral neuropathy. Acta Neuropathol Commun 1:39 (2013). Mouse . Read more (PubMed: 24252174) »
See all 3 Publications for this product

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (AGS gastric epithelial cell line)
Gel Running Conditions
Reduced Denaturing (SDS-PAGE (9.5% AA gel))
Loading amount
80 µg
Treatment
cultured in RPMI with 10% FBS
Specification
AGS gastric epithelial cell line
Blocking step
TBS-Tween with 5%Milk & 0.5%BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Sep 07 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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