Key features and details
- Rabbit polyclonal to ERC2
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-ERC2 antibody
See all ERC2 primary antibodies
DescriptionRabbit polyclonal to ERC2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Rat ERC2 aa 650-750 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Rat brain tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab27250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionThought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act together with BSN. May recruit liprin-alpha proteins to the CAZ.
Cellular localizationCytoplasm. Cell junction > synapse. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. In neurons, localized to synapses, and colocalizes with PCLO. Localized to the active zone of presynaptic density.
- Information by UniProt
- Cast antibody
- CAST1 antibody
- CAZ-associated structural protein 1 antibody
Anti-ERC2 antibody (ab27250) at 1 µg/ml + Rat Brain Whole Tissue Lysate at 20 µg
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 111 kDa
Observed band size: 111 kDa
Additional bands at: 50 kDa (possible non-specific secondary antibody binding)
Image courtesy of Human Protein Atlas
ab27250 staining ERC2 in Human cerebellum. The paraffin embedded human tissue was incubated with ab27250 (1/50 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab27250 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
ab27250 has not yet been referenced specifically in any publications.