• Product name
    Anti-ERCC1 antibody [3H11]
    See all ERCC1 primary antibodies
  • Description
    Mouse monoclonal [3H11] to ERCC1
  • Host species
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    His-tagged recombinant human ERCC1 (excision repair cross complementing) protein.

  • Positive control
    • A431 or HeLa cells.



Our Abpromise guarantee covers the use of ab43 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2 µg/mg of lysate.
WB 1/375 - 1/750. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).


  • Function
    Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
  • Involvement in disease
    Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
  • Sequence similarities
    Belongs to the ERCC1/RAD10/SWI10 family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • COFS 4 antibody
    • COFS4 antibody
    • DNA excision repair protein ERCC 1 antibody
    • DNA excision repair protein ERCC-1 antibody
    • DNA excision repair protein ERCC1 antibody
    • ERCC 1 antibody
    • ERCC1 antibody
    • ERCC1_HUMAN antibody
    • Excision repair cross complementation group 1 antibody
    • Excision repair cross complementing 1 antibody
    • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
    • Excision repair protein antibody
    • RAD 10 antibody
    • RAD10 antibody
    • UV 20 antibody
    • UV20 antibody
    see all


This product has been referenced in:
  • Prewett M  et al. Tumors established with cell lines selected for oxaliplatin resistance respond to oxaliplatin if combined with cetuximab. Clin Cancer Res 13:7432-40 (2007). WB ; Human . Read more (PubMed: 18094427) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us.

The lab has contacted me today regarding ab43. They have said that the information they gave me yesterday was wrong and that this antibody has indeed been tested in IP and therefore is guaranteed to function. Read More

Thank you for contacting us.

I have contacted the lab regarding the protocol used to do IP for ab43. Unfortunately, I have found out that we have no information regarding how this antibody functions in IP and therefore we cannot guarantee th...

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Thank you for your reply and for kindly providing the further information.

The following secondaries detect mouse IgG2a, and are tested in WB and conjugated to HRP. I can recommend to review the online datasheets for further information. Read More

Thank you for your enquiry.

I can confirm that ab43ERCC1 antibody [3H11] is a mouse monoclonal,IgG2a isotype.

The ab97240 secondary antibody detects mouse IgG1 isotype, so it would not be suitable for use with ab43. You will need ...

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Thank you for your enquiry. To our knowledge, ab43 has not yet been tested for application in IF. The antibody was originally characterized for use in Western blotting and IP. We have another ERCC1 antibody that hasn't been tested yet in IF either,...

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Human ERCC1 is approximately 32kDa.


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