Anti-ERCC1 antibody (ab196869)
Key features and details
- Rabbit polyclonal to ERCC1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ERCC1 antibody
See all ERCC1 primary antibodies -
Description
Rabbit polyclonal to ERCC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein within Human ERCC1. The exact sequence is proprietary.
Database link: P07992 -
Positive control
- Raji, MCF7, PC3, HepG2, HeLa, Mouse liver and Mouse brain cell line extracts; Human kidney and Human kidney cancer tissues; U2OS cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
(without Mg2+ and Ca2+) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 33 kDa.
|
|
ICC/IF |
1/50 - 1/200.
|
|
IHC-P |
1/50 - 1/200.
|
Notes |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 33 kDa. |
ICC/IF
1/50 - 1/200. |
IHC-P
1/50 - 1/200. |
Target
-
Function
Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. -
Involvement in disease
Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. -
Sequence similarities
Belongs to the ERCC1/RAD10/SWI10 family. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 2067 Human
- Entrez Gene: 13870 Mouse
- Omim: 126380 Human
- SwissProt: P07992 Human
- SwissProt: P07903 Mouse
- Unigene: 435981 Human
- Unigene: 280913 Mouse
-
Alternative names
- COFS 4 antibody
- COFS4 antibody
- DNA excision repair protein ERCC 1 antibody
see all
Images
-
All lanes : Anti-ERCC1 antibody (ab196869) at 1/500 dilution
Lane 1 : Raji cell line extract
Lane 2 : MCF7 cell line extract
Lane 3 : PC3 cell line extract
Lane 4 : HepG2 cell line extract
Lane 5 : HeLa cell line extract
Lane 6 : Mouse liver tissue extract
Lane 7 : Mouse brain tissue extract
Predicted band size: 33 kDa -
Immunofluorescent analysis of U2OS cells labeling ERCC1 with ab196869 at 1/50. Image at the bottom costained with DAPI.
-
Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissue labeling ERCC1 with ab196869 at 1/50.
-
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERCC1 with ab196869 at 1/50.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab196869 has not yet been referenced specifically in any publications.