Overview

  • Product name

  • Description

    Rabbit polyclonal to ERCC1
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous level of total ERCC1 protein.
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein within Human ERCC1. The exact sequence is proprietary.
    Database link: P07992

  • Positive control

    • Raji, MCF7, PC3, HepG2, HeLa, Mouse liver and Mouse brain cell line extracts; Human kidney and Human kidney cancer tissues; U2OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab196869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 33 kDa.
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/200.

Target

  • Function

    Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
  • Involvement in disease

    Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
  • Sequence similarities

    Belongs to the ERCC1/RAD10/SWI10 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • COFS 4 antibody
    • COFS4 antibody
    • DNA excision repair protein ERCC 1 antibody
    • DNA excision repair protein ERCC-1 antibody
    • DNA excision repair protein ERCC1 antibody
    • ERCC 1 antibody
    • ERCC1 antibody
    • ERCC1_HUMAN antibody
    • Excision repair cross complementation group 1 antibody
    • Excision repair cross complementing 1 antibody
    • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
    • Excision repair protein antibody
    • RAD 10 antibody
    • RAD10 antibody
    • UV 20 antibody
    • UV20 antibody
    see all

Images

  • All lanes : Anti-ERCC1 antibody (ab196869) at 1/500 dilution

    Lane 1 : Raji cell line extract
    Lane 2 : MCF7 cell line extract
    Lane 3 : PC3 cell line extract
    Lane 4 : HepG2 cell line extract
    Lane 5 : HeLa cell line extract
    Lane 6 : Mouse liver tissue extract
    Lane 7 : Mouse brain tissue extract

    Predicted band size: 33 kDa

  • Immunofluorescent analysis of U2OS cells labeling ERCC1 with ab196869 at 1/50. Image at the bottom costained with DAPI.

  • Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissue labeling ERCC1 with ab196869 at 1/50.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERCC1 with ab196869 at 1/50.

References

ab196869 has not yet been referenced specifically in any publications.

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