Key features and details
- Rabbit polyclonal to ERCC1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ERCC1 antibody
See all ERCC1 primary antibodies
DescriptionRabbit polyclonal to ERCC1
SpecificityThis antibody detects endogenous level of total ERCC1 protein.
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein within Human ERCC1. The exact sequence is proprietary.
Database link: P07992
- Raji, MCF7, PC3, HepG2, HeLa, Mouse liver and Mouse brain cell line extracts; Human kidney and Human kidney cancer tissues; U2OS cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
(without Mg2+ and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 33 kDa.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/200.|
FunctionStructure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
Involvement in diseaseDefects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
Sequence similaritiesBelongs to the ERCC1/RAD10/SWI10 family.
- Information by UniProt
- COFS 4 antibody
- COFS4 antibody
- DNA excision repair protein ERCC 1 antibody
All lanes : Anti-ERCC1 antibody (ab196869) at 1/500 dilution
Lane 1 : Raji cell line extract
Lane 2 : MCF7 cell line extract
Lane 3 : PC3 cell line extract
Lane 4 : HepG2 cell line extract
Lane 5 : HeLa cell line extract
Lane 6 : Mouse liver tissue extract
Lane 7 : Mouse brain tissue extract
Predicted band size: 33 kDa
Immunofluorescent analysis of U2OS cells labeling ERCC1 with ab196869 at 1/50. Image at the bottom costained with DAPI.
Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissue labeling ERCC1 with ab196869 at 1/50.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERCC1 with ab196869 at 1/50.
ab196869 has not yet been referenced specifically in any publications.