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  1. Link

    ercc1-antibody-ab227286.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Nucl. Excision Repair
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Anti-ERCC1 antibody (ab227286)

  • Datasheet
  • SDS
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Western blot - Anti-ERCC1 antibody (ab227286)
  • Western blot - Anti-ERCC1 antibody (ab227286)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody (ab227286)

Key features and details

  • Rabbit polyclonal to ERCC1
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human ERCC1 protein (ab126690)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ERCC1 antibody
    See all ERCC1 primary antibodies
  • Description

    Rabbit polyclonal to ERCC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment within Human ERCC1 (internal sequence). The exact sequence is proprietary.
    Database link: P07992

  • Positive control

    • WB: HeLa cell extract. ICC/IF: A549 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Nucl. Excision Repair
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human ERCC1 protein (ab126690)

Applications

Our Abpromise guarantee covers the use of ab227286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000.

Target

  • Function

    Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
  • Involvement in disease

    Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
  • Sequence similarities

    Belongs to the ERCC1/RAD10/SWI10 family.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P07992 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 450179 Chimpanzee
    • Entrez Gene: 2067 Human
    • Omim: 126380 Human
    • SwissProt: P07992 Human
    • Unigene: 435981 Human
    • Alternative names

      • COFS 4 antibody
      • COFS4 antibody
      • DNA excision repair protein ERCC 1 antibody
      • DNA excision repair protein ERCC-1 antibody
      • DNA excision repair protein ERCC1 antibody
      • ERCC 1 antibody
      • ERCC1 antibody
      • ERCC1_HUMAN antibody
      • Excision repair cross complementation group 1 antibody
      • Excision repair cross complementing 1 antibody
      • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
      • Excision repair protein antibody
      • RAD 10 antibody
      • RAD10 antibody
      • UV 20 antibody
      • UV20 antibody
      see all

    Images

    • Western blot - Anti-ERCC1 antibody (ab227286)
      Western blot - Anti-ERCC1 antibody (ab227286)
      All lanes : Anti-ERCC1 antibody (ab227286) at 1/1000 dilution

      Lane 1 : WT HeLa cell extract
      Lane 2 : ERCC1 Knockout HeLa cell extracts

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG
    • Western blot - Anti-ERCC1 antibody (ab227286)
      Western blot - Anti-ERCC1 antibody (ab227286)
      All lanes : Anti-ERCC1 antibody (ab227286) at 1/1000 dilution

      Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : ERCC1 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG


      10% SDS-PAGE gel.

    • Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody (ab227286)
      Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody (ab227286)

      A549 (human lung carcinoma cell line) cells stained for ERCC1 (green) using ab227286 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Nuclear counterstain: Hoechst 33342 (blue).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab227286? Please let us know so that we can cite the reference in this datasheet.

    ab227286 has not yet been referenced specifically in any publications.

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