Anti-ERCC1 antibody (ab227286)
Key features and details
- Rabbit polyclonal to ERCC1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERCC1 antibody
See all ERCC1 primary antibodies -
Description
Rabbit polyclonal to ERCC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Recombinant fragment within Human ERCC1 (internal sequence). The exact sequence is proprietary.
Database link: P07992 -
Positive control
- WB: HeLa cell extract. ICC/IF: A549 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. |
Target
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Function
Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. -
Involvement in disease
Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. -
Sequence similarities
Belongs to the ERCC1/RAD10/SWI10 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 450179 Chimpanzee
- Entrez Gene: 2067 Human
- Omim: 126380 Human
- SwissProt: P07992 Human
- Unigene: 435981 Human
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Alternative names
- COFS 4 antibody
- COFS4 antibody
- DNA excision repair protein ERCC 1 antibody
see all
Images
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Western blot - Anti-ERCC1 antibody (ab227286)All lanes : Anti-ERCC1 antibody (ab227286) at 1/1000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : ERCC1 Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG -
Western blot - Anti-ERCC1 antibody (ab227286)All lanes : Anti-ERCC1 antibody (ab227286) at 1/1000 dilution
Lane 1 : WT HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : ERCC1 knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody (ab227286)A549 (human lung carcinoma cell line) cells stained for ERCC1 (green) using ab227286 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227286 has not yet been referenced specifically in any publications.
