Overview

  • Product name

  • Description

    Goat polyclonal to ERCC1
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human ERCC1 aa 172-184 (internal sequence) (Cysteine residue). (NP_973730.1; NP_001974.1; NP_001159521.1)
    Sequence:

    C-QVDVKDPQQALKE


    Database link: P07992

  • Positive control

    • ICC/IF: A431 and U-2 OS cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
  • Involvement in disease

    Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
  • Sequence similarities

    Belongs to the ERCC1/RAD10/SWI10 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • COFS 4 antibody
    • COFS4 antibody
    • DNA excision repair protein ERCC 1 antibody
    • DNA excision repair protein ERCC-1 antibody
    • DNA excision repair protein ERCC1 antibody
    • ERCC 1 antibody
    • ERCC1 antibody
    • ERCC1_HUMAN antibody
    • Excision repair cross complementation group 1 antibody
    • Excision repair cross complementing 1 antibody
    • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
    • Excision repair protein antibody
    • RAD 10 antibody
    • RAD10 antibody
    • UV 20 antibody
    • UV20 antibody
    see all

Images

  • Paraformaldehyde-fixed, 0.15% Triton permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ERCC1 (Green) using ab238257 at 10 μg/mL in ICC/IF. Primary incubation for 1hour followed by Alexa Fluor®488 secondary antibody (2 μg/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). 

    Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor®488 secondary antibody (2 μg/ml).

  • Paraformaldehyde-fixed, 0.15% Triton permeabilized A431 (human epidermoid carcinoma cell line) cells stained for ERCC1 (Green) using ab238257 at 10 μg/mL in ICC/IF. Primary incubation for 1 hour followed by Alexa Fluor®488 secondary antibody (2 μg/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red). 

    Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor®488 secondary antibody (2 μg/ml).

References

ab238257 has not yet been referenced specifically in any publications.

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