Product nameAnti-ERCC1 antibody [EP2143Y]
See all ERCC1 primary antibodies
DescriptionRabbit monoclonal [EP2143Y] to ERCC1
Specificityab76236 might also detect a heterodimer composed of ERCC1 and XPF/ERCC4 at 100-150 kDa.
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human ERCC1 aa 1-100 (N terminal). The exact sequence is proprietary.
- SKBR-3 cell lysate
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa).|
|Flow Cyt||1/20 - 1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionStructure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
Involvement in diseaseDefects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
Sequence similaritiesBelongs to the ERCC1/RAD10/SWI10 family.
- Information by UniProt
- COFS 4 antibody
- COFS4 antibody
- DNA excision repair protein ERCC 1 antibody
Flow Cytometry analysis of SK-BR-3 (Human breast adenocarcinoma epithelial cell) cells labeling ERCC1 with purified ab76326 at 1/20 dilution (12.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
Anti-ERCC1 antibody [EP2143Y] (ab76236) at 1/2000 dilution + SKBR-3 cell lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
The 100 kDa band may represent a heterodimer composed of ERCC1 and XPF/ERCC4.
ab76236 has not yet been referenced specifically in any publications.