Overview

  • Product name

    Anti-ERCC1 antibody [EP2143Y]
    See all ERCC1 primary antibodies
  • Description

    Rabbit monoclonal [EP2143Y] to ERCC1
  • Host species

    Rabbit
  • Specificity

    ab76236 might also detect a heterodimer composed of ERCC1 and XPF/ERCC4 at 100-150 kDa.
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ERCC1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • SKBR-3 cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab76236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 32 kDa).
Flow Cyt 1/20 - 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
    • Involvement in disease

      Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
    • Sequence similarities

      Belongs to the ERCC1/RAD10/SWI10 family.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • COFS 4 antibody
      • COFS4 antibody
      • DNA excision repair protein ERCC 1 antibody
      • DNA excision repair protein ERCC-1 antibody
      • DNA excision repair protein ERCC1 antibody
      • ERCC 1 antibody
      • ERCC1 antibody
      • ERCC1_HUMAN antibody
      • Excision repair cross complementation group 1 antibody
      • Excision repair cross complementing 1 antibody
      • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
      • Excision repair protein antibody
      • RAD 10 antibody
      • RAD10 antibody
      • UV 20 antibody
      • UV20 antibody
      see all

    Images

    • Flow Cytometry analysis of SK-BR-3 (Human breast adenocarcinoma epithelial cell) cells labeling ERCC1 with purified ab76326 at 1/20 dilution (12.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

    • Anti-ERCC1 antibody [EP2143Y] (ab76236) at 1/2000 dilution + SKBR-3 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 32 kDa
      Observed band size: 36 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.



      The 100 kDa band may represent a heterodimer composed of ERCC1 and XPF/ERCC4.

    References

    ab76236 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab76236.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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