RecombinantRabMAb

Recombinant Anti-ERCC1 antibody [EPR7062] (ab129093)

Submit a review Q&A (1)
Western blot - Anti-ERCC1 antibody [EPR7062] (ab129093)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [EPR7062] (ab129093)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [EPR7062] (ab129093)
  • Anti-ERCC1 antibody [EPR7062] (ab129093)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7062] to ERCC1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ERCC1 antibody [EPR7062]
    See all ERCC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7062] to ERCC1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human ERCC1 aa 150-300. The exact sequence is proprietary.
    Database link: P07992

  • Positive control

    • HeLa, SKBR-3, and 293T cell lysates, Human lung carcinoma tissue, HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab129093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.

    • Involvement in disease

      Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.

    • Sequence similarities

      Belongs to the ERCC1/RAD10/SWI10 family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • COFS 4 antibody
        • COFS4 antibody
        • DNA excision repair protein ERCC 1 antibody
        • DNA excision repair protein ERCC-1 antibody
        • DNA excision repair protein ERCC1 antibody
        • ERCC 1 antibody
        • ERCC1 antibody
        • ERCC1_HUMAN antibody
        • Excision repair cross complementation group 1 antibody
        • Excision repair cross complementing 1 antibody
        • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
        • Excision repair protein antibody
        • RAD 10 antibody
        • RAD10 antibody
        • UV 20 antibody
        • UV20 antibody
        see all

      Images

      • Western blot - Anti-ERCC1 antibody [EPR7062] (ab129093)
        Western blot - Anti-ERCC1 antibody [EPR7062] (ab129093)
        All lanes : Anti-ERCC1 antibody [EPR7062] (ab129093) at 1/1000 dilution

        Lane 1 : HeLa cell lysates
        Lane 2 : SKBR-3 cell lysates
        Lane 3 : 293T cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 33 kDa
        Observed band size: 36 kDa
        why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [EPR7062] (ab129093)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [EPR7062] (ab129093)

        Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for ERCC1 with ab129093 (1/25 dilution) in immunohistochemical analysis.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [EPR7062] (ab129093)
        Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [EPR7062] (ab129093)
        ab129093, at a 1/100 dilution, staining ERCC1 in HeLa cells by Immunofluorescence.
      • Anti-ERCC1 antibody [EPR7062] (ab129093)
        Anti-ERCC1 antibody [EPR7062] (ab129093)

      References (0)

      Publishing research using ab129093? Please let us know so that we can cite the reference in this datasheet.

      ab129093 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Question

      I am looking for an antibody against mouse Ercc1 that is not raised against the C-terminus of the protein.

      Therefore I was wondering to which part of the Ercc1 protein your antibodies ab129093 [EPR7062] and ab129267 [EPR7277] are raised or are they against the full-length?

      Kind regards,

      Read More
      Answer

      Thank you for contacting us.

      I can confirm, that the ab129267 is raised against the first 160 aa and binds to the N-Terminus of the ERCC1 protein.

      The ab129093 is regrettably raised against the C-terminus of the ERCC1 protein and for that reason unlikely to be suitable for your purpose.


      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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