Overview

  • Product name

    Anti-ERCC1 antibody [EPR7277]
    See all ERCC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7277] to ERCC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ERCC1 aa 1-200. The exact sequence is proprietary.
    Database link: P07992

  • Positive control

    • Human lung carcinoma tissue; HeLa, HepG2 and SKBR-3 cell lysates. HepG2 cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
    • Involvement in disease

      Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
    • Sequence similarities

      Belongs to the ERCC1/RAD10/SWI10 family.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • COFS 4 antibody
      • COFS4 antibody
      • DNA excision repair protein ERCC 1 antibody
      • DNA excision repair protein ERCC-1 antibody
      • DNA excision repair protein ERCC1 antibody
      • ERCC 1 antibody
      • ERCC1 antibody
      • ERCC1_HUMAN antibody
      • Excision repair cross complementation group 1 antibody
      • Excision repair cross complementing 1 antibody
      • Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
      • Excision repair protein antibody
      • RAD 10 antibody
      • RAD10 antibody
      • UV 20 antibody
      • UV20 antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for ERCC1 with ab129267 (1/100 dilution) in immunohistochemical analysis.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab129267, at 1/100 dilution, staining ERCC1 in HepG2 cells by Immunofluorescence.
    • All lanes : Anti-ERCC1 antibody [EPR7277] (ab129267) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SKBR-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP

      Predicted band size: 33 kDa
      Observed band size: 36 kDa
      why is the actual band size different from the predicted?

    • Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling ERCC1 with purified ab129267 at 1/80 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    References

    This product has been referenced in:

    • Brown KM  et al. Using patient-derived xenograft models of colorectal liver metastases to predict chemosensitivity. J Surg Res 227:158-167 (2018). Read more (PubMed: 29804848) »
    • Anurag M  et al. Comprehensive Profiling of DNA Repair Defects in Breast Cancer Identifies a Novel Class of Endocrine Therapy Resistance Drivers. Clin Cancer Res 24:4887-4899 (2018). Read more (PubMed: 29793947) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (lung - lung adenocarcinoma)
    Specification
    lung - lung adenocarcinoma

    Dr. Mladen Jokic

    Verified customer

    Submitted Oct 27 2014

    Answer

    Thank you for contacting us.

    I can confirm, that the ab129267 is raised against the first 160 aa and binds to the N-Terminus of the ERCC1 protein.

    The ab129093 is regrettably raised against the C-terminus of the ERCC1 protein and for that reason unlikely to be suitable for your purpose.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question
    Answer

    Thank you for calling earlier today.

    1. For a hematoxylin stain for nuclei, you will need your hematoxylin solution, which I am assuming is ready-to-use, and a 1% solution of "acid alcohol". This is prepared by simply adding 1 ml of HCL to 99 ml of 70% ethanol. (Use full-strength, 37%, HCl also referred to as 12N. Be careful with this! Use only in a fume hood).

    2. Incubate the sections in the hematoxylin only after they have been stained with DAB and rinsed in distilled water. 2 minutes incubation in the hematoxylin should be sufficient but it will depend on the type of hematoxylin you have. In any case, you will not need to incubate for more than 8 minutes, room temperature.

    3. Rinse in two changes of distilled water, then dip in acid alcohol solution two or three times to remove most of the stain. The nuclei should remain stained, and there may be a light overall blue stain of the rest of the section, which is OK. If there seems to be too much, you can keep dipping in the acid alcohol to remove more.

    4. Rinse in running tap water for two minutes. This needs to be tap water, not distilled, as the ions in the tap water will help develop a nice blue shade.

    5. Now you can coverslip with your aqueous mounting medium! You can practice with sections that you have not stained for ERCC1 beforehand, to make sure you get a counterstain you like. If the hematoxylin is old, it may create problems, like splotchy staining.

    Read More
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Specification
    Tonsil
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 9
    Permeabilization
    Yes - Triton x100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10%

    Abcam user community

    Verified customer

    Submitted Aug 13 2012

    Question
    Answer

    Merci de nous avoir contactés.

    Merci de nous avoir confirmé que le ab129267 fonctionne bien dans vos mains. Nous nous rejouissons aussi de recevoir l'Abreview avec vos résultats.

    Je me permets de vous transférer le numéro de commande del'envoie de la deuxième unité xxx . Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

    N'hésitez pas à nous contacter lors d'une prochaine occasion.

    Read More

    Answer

    ro

    Je suis heureuse d'entendre que cet anticorps vous donne des bons résultats!

    J'ai discuté avec mes collègues, et puisque vous avez eu autant de difficultés avec nos anticorps contre l'ERCC1, nous sommes d'accord de faire un geste commercial et vous envoyer une unité gratuite de ce produit si vous n'avez pas assez pour finir vos études. Est-ce que vous pourriez aussi nous envoyer un feedback sur cette anticorps par un Abreview? Nous vous serions très reconnaissants. Je vous invite d'ailleurs de soumettre des feedback sur l'utilisation pour tous nos produits. Pour savoir plus sur les feedbacks, veuillez consulter le site suivant: https://www.abcam.com/abreview

    Veuillez s'il vous plaît nous tenir au courant de votre progrès, afin quenouspouvons arrangerl'envoie d'une deuxième unité de l'ab129267 si souhaité.

    J'espère que ceci vous convient. Je me tiens à votre disposition pour d'autres questions.

    Read More

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