Recombinant Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Product name

Anti-ERCC1 antibody [SP68] - BSA and Azide free
See all ERCC1 primary antibodies
Description

Rabbit monoclonal [SP68] to ERCC1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-Pmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Cow, Pig
Immunogen

Recombinant full length protein corresponding to Human ERCC1.
Database link: P07992
Positive control
- IHC-P: Human lung carcinoma, bladder transitional cell carcinoma, testis, breast ductal carcinoma, colon, colon carcinoma, hepatocellular carcinoma, lung carcinoma, prostate carcinoma, renal cell carcinoma, skin squamous cell carcinoma, Mouse colon, and Rat colon tissue.
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Ab236442 is the carrier-free version of ab225721. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

ab236442 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP68
Isotype

IgG
Research areas

Alternative Versions
- Anti-ERCC1 antibody [SP68] (ab225721)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Conjugation kits
- FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human ERCC1 protein (ab126690)

Our Abpromise guarantee covers the use of ab236442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 30 minutes at room temperature.

Function

Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
Involvement in disease

Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur.
Sequence similarities

Belongs to the ERCC1/RAD10/SWI10 family.
Cellular localization

Nucleus.
Target information above from: UniProt accession P07992 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 615637 Cow
- Entrez Gene: 2067 Human
- Entrez Gene: 13870 Mouse
- Entrez Gene: 100286808 Pig
- Entrez Gene: 292673 Rat
- Omim: 126380 Human
- SwissProt: Q1LZ75 Cow
- SwissProt: P07992 Human
- SwissProt: P07903 Mouse
- Unigene: 435981 Human
- Unigene: 280913 Mouse
see all
Alternative names
- COFS 4 antibody
- COFS4 antibody
- DNA excision repair protein ERCC 1 antibody
- DNA excision repair protein ERCC-1 antibody
- DNA excision repair protein ERCC1 antibody
- ERCC 1 antibody
- ERCC1 antibody
- ERCC1_HUMAN antibody
- Excision repair cross complementation group 1 antibody
- Excision repair cross complementing 1 antibody
- Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody
- Excision repair protein antibody
- RAD 10 antibody
- RAD10 antibody
- UV 20 antibody
- UV20 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling ERCC1 with Purified ab225721 at 1/400 dilution (0.132 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling ERCC1 with Purified ab225721 at 1/400 dilution (0.132 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling ERCC1 with Purified ab225721 at 1/100 dilution (0.53 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human testis tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human colon tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab225721).
Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet

Publishing research using ab236442? Please let us know so that we can cite the reference in this datasheet.

ab236442 has not yet been referenced specifically in any publications.

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Carrier free

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

Applications

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (0)

Customer reviews and Q&As