• Product name
  • Description
    Mouse monoclonal to ERCC6L
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-420 of Human FLJ20105 protein

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab57434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


  • Function
    DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 TPR repeats.
  • Post-translational
    Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.
  • Cellular localization
    Chromosome > centromere. Chromosome > centromere > kinetochore. Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase ERCC6 like antibody
    • ATP-dependent helicase ERCC6-like antibody
    • DNA excision repair protein ERCC 6 like antibody
    • DNA excision repair protein ERCC-6-like antibody
    • ERC6L_HUMAN antibody
    • ERCC 6L antibody
    • Ercc6l antibody
    • Excision repair cross complementing rodent repair deficiency complementation group 6 like antibody
    • Excision repair protein ERCC6 like antibody
    • FLJ20105 antibody
    • MGC131695 antibody
    • PICH antibody
    • Plk1 interacting checkpoint helicase antibody
    • PLK1-interacting checkpoint helicase antibody
    • SNF2/RAD54 family protein antibody
    • Tumor antigen BJ HCC 15 antibody
    • Tumor antigen BJ-HCC-15 antibody
    see all


  • Western blot against tagged recombinant protein immunogen using ab57434 FLJ20105 protein antibody at 1ug/ml. Predicted band size of immunogen is 72 kDa


This product has been referenced in:
  • Nielsen CF  et al. PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis. Nat Commun 6:8962 (2015). Read more (PubMed: 26643143) »

See 1 Publication for this product

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