Overview

  • Product name

    Anti-ERCC8 antibody [EPR9237]
    See all ERCC8 primary antibodies
  • Description

    Rabbit monoclonal [EPR9237] to ERCC8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human ERCC8 (UniProt ID: Q13216)

  • Positive control

    • HeLa, Molt-4, 293T, and HepG2 whole cell lysate (ab7900).
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9237
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitement of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes.
    • Pathway

      Protein modification; protein ubiquitination.
    • Involvement in disease

      Defects in ERCC8 are the cause of Cockayne syndrome type A (CSA) [MIM:216400]. Cockayne syndrome is a rare disorder characterized by cutaneous sensitivity to sunlight, abnormal and slow growth, cachectic dwarfism, progeroid appearance, progressive pigmentary retinopathy and sensorineural deafness. There is delayed neural development and severe progressive neurologic degeneration resulting in mental retardation. Two clinical forms are recognized: in the classical form or Cockayne syndrome type 1, the symptoms are progressive and typically become apparent within the first few years or life; the less common Cockayne syndrome type 2 is characterized by more severe symptoms that manifest prenatally. Cockayne syndrome shows some overlap with certain forms of xeroderma pigmentosum. Unlike xeroderma pigmentosum, patients with Cockayne syndrome do not manifest increased freckling and other pigmentation abnormalities in the skin and have no significant increase in skin cancer.
    • Sequence similarities

      Contains 5 WD repeats.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CKN1 antibody
      • Cockayne syndrome type A antibody
      • Cockayne syndrome WD repeat protein CSA antibody
      • CSA antibody
      • DNA excision repair protein ERCC-8 antibody
      • DNA excision repair protein ERCC8 antibody
      • ERCC 8 antibody
      • ERCC8 antibody
      • ERCC8_HUMAN antibody
      • excision repair cross-complementing rodent repair deficiency, complementation group 8 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: ERCC8 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Molt-4 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab137033 observed at 44 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab137033 was shown to recognize ERCC8 when ERCC8 knockout samples were used, along with additional cross-reactive bands. Wild-type and ERCC8 knockout samples were subjected to SDS-PAGE. ab137033 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively, and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-ERCC8 antibody [EPR9237] (ab137033) at 1/1000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : Molt 4 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 44 kDa

    References

    This product has been referenced in:

    • Wienholz F  et al. FACT subunit Spt16 controls UVSSA recruitment to lesion-stalled RNA Pol II and stimulates TC-NER. Nucleic Acids Res N/A:N/A (2019). Read more (PubMed: 30715484) »
    • Slyskova J  et al. Base and nucleotide excision repair facilitate resolution of platinum drugs-induced transcription blockage. Nucleic Acids Res 46:9537-9549 (2018). Read more (PubMed: 30137419) »
    See all 4 Publications for this product

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