Overview

  • Product name

    Anti-EREG antibody - C-terminal
    See all EREG primary antibodies
  • Description

    Rabbit polyclonal to EREG - C-terminal
  • Host species

    Rabbit
  • Specificity

    ab175118 detects the C-terminal propeptide of EREG, not the mature form.
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human EREG aa 140-169 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O14944

  • Positive control

    • HepG2 whole cell lysate (ab7900), Human colon carcinoma tissue, HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab175118 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.
  • Tissue specificity

    In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon.
  • Sequence similarities

    Contains 1 EGF-like domain.
  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epiregulin antibody
    • EPR antibody
    • ER antibody
    • Ereg antibody
    • EREG_HUMAN antibody
    • Proepiregulin antibody
    see all

Images

  • Anti-EREG antibody - C-terminal (ab175118) at 1/100 dilution + HepG2 cell lysate at 35 µg

    Predicted band size: 19 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue, labeling EREG with ab175118 at 1/10 dilution. IHC was followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of HepG2 cells, labeling EREG with ab175118 at 1/10 diulution (right histogram; green), compared with a negative control cell (left histogram; blue).

References

This product has been referenced in:

  • Pradhan SA  et al. Evidence that TSC2 acts as a transcription factor and binds to and represses the promoter of Epiregulin. Nucleic Acids Res 42:6243-55 (2014). WB ; Human . Read more (PubMed: 24748662) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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