Overview

  • Product name

    Anti-EREG antibody [MM0265- 12Y4]
    See all EREG primary antibodies
  • Description

    Mouse monoclonal [MM0265- 12Y4] to EREG
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length Human EREG protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    MM0265- 12Y4
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.

Target

  • Function

    May be a mediator of localized cell proliferation. As a mitogen it may stimulate cell proliferation and/or angiogenesis.
  • Tissue specificity

    In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon.
  • Sequence similarities

    Contains 1 EGF-like domain.
  • Cellular localization

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Epiregulin antibody
    • EPR antibody
    • ER antibody
    • Ereg antibody
    • EREG_HUMAN antibody
    • Proepiregulin antibody
    see all

References

This product has been referenced in:

  • Oliveras-Ferraros C  et al. Cross-suppression of EGFR ligands amphiregulin and epiregulin and de-repression of FGFR3 signalling contribute to cetuximab resistance in wild-type KRAS tumour cells. Br J Cancer 106:1406-14 (2012). Read more (PubMed: 22491422) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your reply. If the white band you are seeing on the darker background is at a molecular weight of 19 kDa, then it is the specific band of interest. The band can show up lighter than the background when the signal is too strong and the ECL reagent is consumed too quickly. To get a better image, you can either switch to a less sensitive ECL, reduce the primary antibody to 1:2500 or even 1:5000, or load less protein. If that does not help, please let me know and I will be happy to offer you a free of charge replacement, credit, or refund.

Read More

Question
Answer

Thank you for contacting us. I am sorry this antibody is not giving good results in WB. Can you please provide some additional details of your experiment?

1) What type of samples were you testing and how were they prepared (lysis buffer, protease inhibitors, reduced / denatured, etc)? How much protein was loaded per lane?

2) Which of the images you sent were produced using ab89291? What molecular weights do the bands correspond to? The inverse bands you observed are generally an indication that the signal is too strong. The image can be improved by reducing the primary and secondary antibody concentrations.

3) What blocking agent were you using?

I will be happy to help you further once I have this additional information

Read More

Answer

Thank you for contacting us and your interest in our products. We have several antibodies directed against amphiregulin and epiregulin, however, as discussed over the phone, none have as yet been tested in IHC.

This does not mean that they would not work. Simply that we have not tested it ourselves and would therefore not be able to guarantee that they would work in this application.

Against amphiregulin I would suggest the rabbit polyclonal https://www.abcam.com/Amphiregulin-antibody-ab33558.htmlto try and for the epiregulin (EREG), the mouse monoclonal https://www.abcam.com/EREG-antibody-MM0265-12Y4-ab89291.html.

As discussed over the phone, if you would like to try either of these antibodies in IHC you would be eligible for our testing discount scheme. This offer means that if you purchase ab33558 and/or ab89291as normal, test the antibody with yourhuman tissue in IHC and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab33558 and/or ab89291 off any product). More information on this offer can be found here:

www.abcam.com/collaborationdiscount

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab33558 and/or ab89291.

As we have not tried either of these antibodies in IHC as yet we do not have a recommended protocol but I can advise that you have a look at the following protocol guide to get an idea of what optimisation steps may be worth trying (including the antigen retrieval that may be necessary):

https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf

I would start with a dilution of 10 ug/mL for the anti-amphiregulin ab33558 and 5 ug/mL of the anti-epiregulin ab89291.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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