Anti-ERF antibody (ab84773)
Key features and details
- Rabbit polyclonal to ERF
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ERF antibody
See all ERF primary antibodies -
Description
Rabbit polyclonal to ERF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to a region between residue 498 and 548 of human ERF (NP_006485.2).
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Positive control
- Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Prostate Carcinoma Mouse Controls: Renal Cell Carcinoma, Teratoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab84773 was affinity purified using an epitope specific to ERF immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Notes |
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Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation. -
Tissue specificity
Highest levels in testis, ovary, pancreas, and heart. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Post-translational
modificationsPhosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 789798 Cow
- Entrez Gene: 2077 Human
- Entrez Gene: 13875 Mouse
- Entrez Gene: 292721 Rat
- Omim: 611888 Human
- SwissProt: P50548 Human
- SwissProt: P70459 Mouse
- Unigene: 655969 Human
see all -
Alternative names
- ERF antibody
- ERF_HUMAN antibody
- ETS domain containing transcription factor ERF antibody
see all
Images
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Staining of ERF by Immunohistochemistry on a formalin fixed, paraffin embedded section of human ovarian tumor using a 1/100 dilution of ab84773. Detection: DAB staining
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Staining of ERF by Immunohistochemistry on a formalin fixed, paraffin embedded section of mouse teratoma using a 1/500 dilution of ab84773. Detection: DAB staining
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab84773 has not yet been referenced specifically in any publications.