Key features and details
- Rabbit polyclonal to ERF
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ERF antibody
See all ERF primary antibodies
DescriptionRabbit polyclonal to ERF
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to a region between residue 498 and 548 of human ERF (NP_006485.2).
- Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Prostate Carcinoma Mouse Controls: Renal Cell Carcinoma, Teratoma
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab84773 was affinity purified using an epitope specific to ERF immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Our Abpromise guarantee covers the use of ab84773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPotent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation.
Tissue specificityHighest levels in testis, ovary, pancreas, and heart.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
modificationsPhosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF.
- Information by UniProt
- ERF antibody
- ERF_HUMAN antibody
- ETS domain containing transcription factor ERF antibody
Staining of ERF by Immunohistochemistry on a formalin fixed, paraffin embedded section of human ovarian tumor using a 1/100 dilution of ab84773. Detection: DAB staining
Staining of ERF by Immunohistochemistry on a formalin fixed, paraffin embedded section of mouse teratoma using a 1/500 dilution of ab84773. Detection: DAB staining
ab84773 has not yet been referenced specifically in any publications.