Overview

  • Product name

  • Description

    Rabbit polyclonal to eRF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1-11 of Human eRF1 (NP_004721).

  • Positive control

    • HeLa cells; Raji whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
  • Sequence similarities

    Belongs to the eukaryotic release factor 1 family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cl1 protein antibody
    • D5S1995 antibody
    • ERF antibody
    • eRF1 antibody
    • ERF1_HUMAN antibody
    • ETF1 antibody
    • Eukaryotic peptide chain release factor subunit 1 antibody
    • Eukaryotic release factor 1 antibody
    • Eukaryotic translation termination factor 1 antibody
    • MGC111066 antibody
    • Polypeptide chain release factor 1 antibody
    • Protein Cl1 antibody
    • RF1 antibody
    • Sup45 (yeast omnipotent suppressor 45) homolog like 1 antibody
    • SUP45L1 antibody
    • TB3 1 antibody
    • TB3-1 antibody
    see all

Images

  • Anti-eRF1 antibody (ab153708) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol fixed HeLa cells, labeling eRF1 using ab153708 at a 1/500 dilution. Lower image costained with Hoerchst 33342.

References

ab153708 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153708.
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