Overview

  • Product name

  • Description

    Rabbit polyclonal to eRF3B/GSPT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 395-444 (GANIKEQSDF CPWYTGLPFI PYLDNLPNFN RSIDGPIRLP IVDKYKDMGT) of Human eRF3B/GSPT2 (NP_060564).

  • Positive control

    • ACHN Cell Lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Involved in translation termination in response to the termination codons UAA, UAG and UGA. May play a role as a potent stimulator of the release factor activity of ETF1. Exhibits GTPase activity, which is ribosome- and ETF1-dependent. May play a role in cell cycle progression. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
  • Tissue specificity

    Highly expressed in IUCC stage II colorectal cancer (CRC).
  • Sequence similarities

    Belongs to the GTP-binding elongation factor family. ERF3 subfamily.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • eRF3b antibody
    • ERF3B_HUMAN antibody
    • Eukaryotic peptide chain release factor GTP-binding subunit ERF3B antibody
    • Eukaryotic peptide chain release factor subunit 3b antibody
    • G1 to S phase transition 2 antibody
    • G1 to S phase transition protein 2 homolog antibody
    • Gspt2 antibody
    • GST2 antibody
    • peptide chain release factor 3 antibody
    see all

Images

  • Anti-eRF3B/GSPT2 antibody (ab103086) at 1 µg/ml + ACHN Cell Lysate at 10 µg

    Predicted band size: 69 kDa



    Gel concentration: 12%

References

ab103086 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab103086.
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