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Rabbit polyclonal to eRF3/GSPT1
Recombinant fragment, corresponding to a region within amino acids 1-347 of Human eRF3/GSPT1 (NP_002085).
A549, HeLa, HepG2 and HCT116 whole cell lysates; Human Breast carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 78% PBS, 20% Glycerol, 1% BSA
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 69 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.
Anti Eukaryotic Release Factor 3a antibody
Anti G1 to S phase transition 1 antibody
Western blot - Anti-eRF3/GSPT1 antibody (ab126090)
Anti-eRF3/GSPT1 antibody (ab126090) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 69 kDa 7.5% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF3/GSPT1 antibody (ab126090)
ab126090 at 1/500 dilution, staining eRF3/GSPT1 in Paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"