Overview

  • Product name
    Anti-eRF3/GSPT1 antibody
    See all eRF3/GSPT1 primary antibodies
  • Description
    Rabbit polyclonal to eRF3/GSPT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human eRF3/GSPT1 aa 190-204 (internal sequence) conjugated to keyhole limpet haemocyanin (Cysteine residue).
    Sequence:

    FETGFEKGGQTREHA


    Database link: P15170

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • WB: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab49878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 55 kDa.
IP Use a concentration of 1 - 2 µg/ml.
ICC/IF Use a concentration of 5 - 10 µg/ml. Paraformadehyde fixed cells

Target

  • Relevance
    Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • 551G9.2 antibody
      • Anti Eukaryotic Release Factor 3a antibody
      • Anti G1 to S phase transition 1 antibody
      • Anti GST1, homolog of yeast antibody
      • eRF 3a antibody
      • eRF3a antibody
      • G1 to S phase transition 1 antibody
      • G1 to S phase transition protein 1 homolog antibody
      • G1 to S phase transition protein antibody
      • GSPT 1 antibody
      • GSPT1 antibody
      • GST 1 antibody
      • GST1 antibody
      • GTP binding protein GST1 HS antibody
      • Transition protein 1 antibody
      see all

    References

    This product has been referenced in:
    • Huang L  et al. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biol 19:4 (2018). Read more (PubMed: 29334995) »
    • Nguyen TV  et al. p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates. Proc Natl Acad Sci U S A 114:3565-3571 (2017). WB ; Human . Read more (PubMed: 28320958) »

    See all 6 Publications for this product

    Publishing research using ab49878? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-eRF3/GSPT1 antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lymphoblast)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    15 µg
    Specification
    Lymphoblast
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Jan 20 2017

    Western blot abreview for Anti-eRF3/GSPT1 antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    1.7e+006 cells
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    Username

    Ms. Luisa Romao

    Verified customer

    Submitted Mar 07 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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