Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab49878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 55 kDa.|
|IP||Use a concentration of 1 - 2 µg/ml.|
|ICC/IF||Use a concentration of 5 - 10 µg/ml. Paraformadehyde fixed cells|
RelevanceTranslation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.
- 551G9.2 antibody
- Anti Eukaryotic Release Factor 3a antibody
- Anti G1 to S phase transition 1 antibody
This product has been referenced in:
- Huang L et al. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biol 19:4 (2018). Read more (PubMed: 29334995) »
- Nguyen TV et al. p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates. Proc Natl Acad Sci U S A 114:3565-3571 (2017). WB ; Human . Read more (PubMed: 28320958) »