Anti-eRF3/GSPT1 antibody (ab49878)
Key features and details
- Rabbit polyclonal to eRF3/GSPT1
- Isotype: IgG
Overview
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Product name
Anti-eRF3/GSPT1 antibody
See all eRF3/GSPT1 primary antibodies -
Description
Rabbit polyclonal to eRF3/GSPT1 -
Host species
Rabbit -
Immunogen
Synthetic peptide corresponding to Human eRF3/GSPT1 aa 190-204 (internal sequence) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Sequence:FETGFEKGGQTREHA
Database link: P15170 -
Positive control
- WB: HeLa whole cell lysate (ab150035).
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General notes
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One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Target
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Relevance
Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA. -
Cellular localization
Cytoplasmic -
Alternative names
- 551G9.2 antibody
- Anti Eukaryotic Release Factor 3a antibody
- Anti G1 to S phase transition 1 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab49878 has been referenced in 9 publications.
- Tateno S et al. Genome-wide screening reveals a role for subcellular localization of CRBN in the anti-myeloma activity of pomalidomide. Sci Rep 10:4012 (2020). PubMed: 32132601
- Huang L et al. Targeting Translation Termination Machinery with Antisense Oligonucleotides for Diseases Caused by Nonsense Mutations. Nucleic Acid Ther 29:175-186 (2019). PubMed: 31070517
- Huang L et al. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biol 19:4 (2018). PubMed: 29334995
- Lu G et al. UBE2G1 governs the destruction of cereblon neomorphic substrates. Elife 7:N/A (2018). PubMed: 30234487
- Nguyen TV et al. p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates. Proc Natl Acad Sci U S A 114:3565-3571 (2017). WB ; Human . PubMed: 28320958
- Geißler V et al. The RNA helicase Ddx5/p68 binds to hUpf3 and enhances NMD of Ddx17/p72 and Smg5 mRNA. Nucleic Acids Res 41:7875-88 (2013). WB ; Human . PubMed: 23788676
- Peixeiro I et al. Interaction of PABPC1 with the translation initiation complex is critical to the NMD resistance of AUG-proximal nonsense mutations. Nucleic Acids Res 40:1160-73 (2012). WB ; Human . PubMed: 21989405
- Kumar GR et al. Importin {alpha}-Mediated Nuclear Import of Cytoplasmic Poly(A) Binding Protein Occurs as a Direct Consequence of Cytoplasmic mRNA Depletion. Mol Cell Biol 31:3113-25 (2011). WB, ICC/IF ; Human . PubMed: 21646427
- Singh G et al. A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. PLoS Biol 6:e111 (2008). IP ; Human . PubMed: 18447585