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    erf3gspt1-antibody-ab49878.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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Anti-eRF3/GSPT1 antibody (ab49878)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (9)

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Key features and details

  • Rabbit polyclonal to eRF3/GSPT1
  • Isotype: IgG

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Anti-Casein Kinase 1 alpha antibody [EPR1961(2)] (ab108296)
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Recombinant Human eRF3/GSPT1 protein (ab132287)
Assay
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Overview

  • Product name

    Anti-eRF3/GSPT1 antibody
    See all eRF3/GSPT1 primary antibodies
  • Description

    Rabbit polyclonal to eRF3/GSPT1
  • Host species

    Rabbit
  • Immunogen

    Synthetic peptide corresponding to Human eRF3/GSPT1 aa 190-204 (internal sequence) conjugated to keyhole limpet haemocyanin (Cysteine residue).
    Sequence:

    FETGFEKGGQTREHA


    Database link: P15170
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HeLa whole cell lysate (ab150035).
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human eRF3/GSPT1 protein (ab132287)
  • Related Products

    • Recombinant Human eRF3/GSPT1 protein (ab132287)

Target

  • Relevance

    Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.
  • Cellular localization

    Cytoplasmic
  • Alternative names

    • 551G9.2 antibody
    • Anti Eukaryotic Release Factor 3a antibody
    • Anti G1 to S phase transition 1 antibody
    • Anti GST1, homolog of yeast antibody
    • eRF 3a antibody
    • eRF3a antibody
    • G1 to S phase transition 1 antibody
    • G1 to S phase transition protein 1 homolog antibody
    • G1 to S phase transition protein antibody
    • GSPT 1 antibody
    • GSPT1 antibody
    • GST 1 antibody
    • GST1 antibody
    • GTP binding protein GST1 HS antibody
    • Transition protein 1 antibody
    see all

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (9)

Publishing research using ab49878? Please let us know so that we can cite the reference in this datasheet.

ab49878 has been referenced in 9 publications.

  • Tateno S  et al. Genome-wide screening reveals a role for subcellular localization of CRBN in the anti-myeloma activity of pomalidomide. Sci Rep 10:4012 (2020). PubMed: 32132601
  • Huang L  et al. Targeting Translation Termination Machinery with Antisense Oligonucleotides for Diseases Caused by Nonsense Mutations. Nucleic Acid Ther 29:175-186 (2019). PubMed: 31070517
  • Huang L  et al. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biol 19:4 (2018). PubMed: 29334995
  • Lu G  et al. UBE2G1 governs the destruction of cereblon neomorphic substrates. Elife 7:N/A (2018). PubMed: 30234487
  • Nguyen TV  et al. p97/VCP promotes degradation of CRBN substrate glutamine synthetase and neosubstrates. Proc Natl Acad Sci U S A 114:3565-3571 (2017). WB ; Human . PubMed: 28320958
  • Geißler V  et al. The RNA helicase Ddx5/p68 binds to hUpf3 and enhances NMD of Ddx17/p72 and Smg5 mRNA. Nucleic Acids Res 41:7875-88 (2013). WB ; Human . PubMed: 23788676
  • Peixeiro I  et al. Interaction of PABPC1 with the translation initiation complex is critical to the NMD resistance of AUG-proximal nonsense mutations. Nucleic Acids Res 40:1160-73 (2012). WB ; Human . PubMed: 21989405
  • Kumar GR  et al. Importin {alpha}-Mediated Nuclear Import of Cytoplasmic Poly(A) Binding Protein Occurs as a Direct Consequence of Cytoplasmic mRNA Depletion. Mol Cell Biol 31:3113-25 (2011). WB, ICC/IF ; Human . PubMed: 21646427
  • Singh G  et al. A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay. PLoS Biol 6:e111 (2008). IP ; Human . PubMed: 18447585

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-eRF3/GSPT1 antibody

Good
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphoblast)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
15 µg
Specification
Lymphoblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 20 2017

Western blot abreview for Anti-eRF3/GSPT1 antibody

Good
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
1.7e+006 cells
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Ms. Luisa Romao

Verified customer

Submitted Mar 07 2012

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