Recombinant Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22908-103] to eRF3/GSPT1
- Suitable for: WB
- Reacts with: Mouse, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-eRF3/GSPT1 antibody [EPR22908-103]
See all eRF3/GSPT1 primary antibodies -
Description
Rabbit monoclonal [EPR22908-103] to eRF3/GSPT1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His tagged human eRF3a recombinant protein, PC-3, HeLa, RAW264.7 and NIH/3T3 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22908-103 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000.
|
Notes |
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WB
1/1000. |
Target
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Function
Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. -
Sequence similarities
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.
Contains 1 tr-type G (guanine nucleotide-binding) domain. - Information by UniProt
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Database links
- Entrez Gene: 2935 Human
- Entrez Gene: 14852 Mouse
- Omim: 139259 Human
- SwissProt: P15170 Human
- SwissProt: Q8R050 Mouse
- Unigene: 528780 Human
- Unigene: 325827 Mouse
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Alternative names
- 551G9.2 antibody
- Anti Eukaryotic Release Factor 3a antibody
- Anti G1 to S phase transition 1 antibody
see all
Images
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All lanes : Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 80 kDa why is the actual band size different from the predicted?This antibody recognizes unidentifiable weak bands below 50kDa
Exposure time: Lanes 1-2: 3 seconds Lanes 3-4: 37 seconds
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution
Lane 1 : His tagged human eRF3a recombinant protein, 10 ng
Lane 2 : His tagged human eRF3b recombinant protein, 10ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 48 kDa why is the actual band size different from the predicted?Exposure time: 15 seconds
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab234433 has not yet been referenced specifically in any publications.