Recombinant
RabMAb

Recombinant Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433)

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Overview

  • Product name

    Anti-eRF3/GSPT1 antibody [EPR22908-103]
    See all eRF3/GSPT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22908-103] to eRF3/GSPT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment within Human eRF3/GSPT1 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P15170

  • Positive control

    • WB: His tagged human eRF3a recombinant protein, PC-3, HeLa, RAW264.7 and NIH/3T3 lysates.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Relevance

      Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA.

    • Cellular localization

      Cytoplasmic

    • Database links

      • Alternative names

        • 551G9.2 antibody
        • Anti Eukaryotic Release Factor 3a antibody
        • Anti G1 to S phase transition 1 antibody
        • Anti GST1, homolog of yeast antibody
        • eRF 3a antibody
        • eRF3a antibody
        • G1 to S phase transition 1 antibody
        • G1 to S phase transition protein 1 homolog antibody
        • G1 to S phase transition protein antibody
        • GSPT 1 antibody
        • GSPT1 antibody
        • GST 1 antibody
        • GST1 antibody
        • GTP binding protein GST1 HS antibody
        • Transition protein 1 antibody
        see all

      Images

      • Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433)
        Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433)
        All lanes : Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution

        Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
        Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
        Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
        Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Observed band size: 80 kDa
        why is the actual band size different from the predicted?



        This antibody recognizes unidentifiable weak bands below 50kDa

        Exposure time: Lanes 1-2: 3 seconds Lanes 3-4: 37 seconds

        Blocking and Diluting Buffer and concentration: 5% NFDM/TBST

      • Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433)
        Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433)
        All lanes : Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution

        Lane 1 : His tagged human eRF3a recombinant protein
        Lane 2 : His tagged human eRF3b recombinant protein, 10ng

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Observed band size: 48 kDa why is the actual band size different from the predicted?



        Exposure time: 15 seconds

        Blocking and Diluting Buffer and concentration: 5% NFDM/TBST

      References

      ab234433 has not yet been referenced specifically in any publications.

      Publishing research using ab234433? Please let us know so that we can cite the reference in this datasheet.

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