Recombinant Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22908-103] to eRF3/GSPT1
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-eRF3/GSPT1 antibody [EPR22908-103]
See all eRF3/GSPT1 primary antibodies -
Description
Rabbit monoclonal [EPR22908-103] to eRF3/GSPT1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human eRF3/GSPT1 aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P15170 -
Positive control
- WB: His tagged human eRF3a recombinant protein, PC-3, HeLa, RAW264.7 and NIH/3T3 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22908-103 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab234433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000. |
Target
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Relevance
Translation termination in eukaryotes is governed by two proteins, belonging to the class-1 (eRF1) and class-2 (eRF3) polypeptide release factors. eRF3/GSPT1 is involved in regulation of mammalian cell growth and belongs to the GTP-binding elongation factor family (ERF3 subfamily). eRF3 catalyzes hydrolysis of GTP to GDP and inorganic phosphate in the ribosome in the absence of mRNA, tRNA, aminoacyl-tRNA and peptidyl-tRNA. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 2935 Human
- Entrez Gene: 14852 Mouse
- Omim: 139259 Human
- SwissProt: P15170 Human
- SwissProt: Q8R050 Mouse
- Unigene: 528780 Human
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Alternative names
- 551G9.2 antibody
- Anti Eukaryotic Release Factor 3a antibody
- Anti G1 to S phase transition 1 antibody
see all
Images
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All lanes : Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 80 kDa why is the actual band size different from the predicted?This antibody recognizes unidentifiable weak bands below 50kDa
Exposure time: Lanes 1-2: 3 seconds Lanes 3-4: 37 seconds
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution
Lane 1 : His tagged human eRF3a recombinant protein
Lane 2 : His tagged human eRF3b recombinant protein, 10ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 48 kDa why is the actual band size different from the predicted?Exposure time: 15 seconds
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab234433 has not yet been referenced specifically in any publications.