Product nameAnti-ERG antibody [EPR3863] - ChIP Grade
See all ERG primary antibodies
DescriptionRabbit monoclonal [EPR3863] to ERG - ChIP Grade
Specificityab110639 also detects Fli1.
Tested applicationsSuitable for: WB, IHC-P, ChIPmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human ERG (N terminal). The exact sequence is proprietary.
- Purchase matching WB positive control:Recombinant Human ERG protein
- WB: Jurkat, HT29, and MCF7 cell lysates. IHC-P: Human spleen and tonsil tissues.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
ChIP Related Products
Our Abpromise guarantee covers the use of ab110639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 55 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ChIP||Use at an assay dependent concentration. PubMed: 22615383|
FunctionTranscriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
Involvement in diseaseDefects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4.
Sequence similaritiesBelongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
Cellular localizationNucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
- D030036I24Rik antibody
- Erg 3 antibody
All lanes : Anti-ERG antibody [EPR3863] - ChIP Grade (ab110639) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HT29 cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDa
ab110639 at 1/100 dilution staining ERG in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.
ab110639 at 1/100 dilution staining ERG in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.
This product has been referenced in:
- Yang L et al. TAK1 regulates endothelial cell necroptosis and tumor metastasis. Cell Death Differ N/A:N/A (2019). Read more (PubMed: 30683914) »
- Pontes-Quero S et al. High mitogenic stimulation arrests angiogenesis. Nat Commun 10:2016 (2019). Read more (PubMed: 31043605) »