Overview

  • Product name

    Anti-ERG antibody [EPR3863] - ChIP Grade
    See all ERG primary antibodies
  • Description

    Rabbit monoclonal [EPR3863] to ERG - ChIP Grade
  • Host species

    Rabbit
  • Specificity

    ab110639 also detects Fli1.
  • Tested applications

    Suitable for: WB, IHC-P, ChIPmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human ERG (N terminal). The exact sequence is proprietary.

  • Positive control

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab110639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration. PubMed: 22615383
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
    • Involvement in disease

      Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
      Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4.
    • Sequence similarities

      Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
      Contains 1 PNT (pointed) domain.
    • Cellular localization

      Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links

    • Alternative names

      • Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
      • D030036I24Rik antibody
      • Erg 3 antibody
      • Erg antibody
      • ERG/EWS fusion gene, included antibody
      • ERG/FUS fusion gene, included antibody
      • ERG/TMPSSR2 fusion gene, included antibody
      • ERG_HUMAN antibody
      • ERG1, included antibody
      • ERG2, included antibody
      • ets related antibody
      • ETS-related gene antibody
      • KCNH2 antibody
      • Oncogene ERG antibody
      • p55 antibody
      • TMPRSS2/ERG fusion antibody
      • transcriptional regulator ERG (transforming protein ERG) antibody
      • Transcriptional regulator ERG antibody
      • Transforming protein ERG antibody
      • v ets avian erythroblastosis virus E26 oncogene antibody
      • v ets avian erythroblastosis virus E26 oncogene related antibody
      • v ets erythroblastosis virus E26 oncogene homolog antibody
      • v ets erythroblastosis virus E26 oncogene like antibody
      • v ets erythroblastosis virus E26 oncogene like isoform 2 antibody
      • v-ets erythroblastosis virus E26 oncogene antibody
      • v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody
      • V-ets erythroblastosis virus E26 oncogene like (Avian), isoform CRA_e antibody
      see all

    Images

    • All lanes : Anti-ERG antibody [EPR3863] - ChIP Grade (ab110639) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HT29 cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 55 kDa

    • ab110639 at 1/100 dilution staining ERG in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.
    • ab110639 at 1/100 dilution staining ERG in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.

    References

    This product has been referenced in:

    See all 13 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (E10.5)
    Specification
    E10.5

    Dr. Sophie Astrof

    Verified customer

    Submitted Jan 31 2017

    Answer

    Thank you very much for your interest in ab110639.

    To our knowledge,ab110639 has not been tested in Mouse. Therefore, I can offer a discount off a future purchase if you buy ab110639 now, test it inMouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 freeprimary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab110639 in Mouse. I will then send a discount code. This code must be issued before purchasing ab110639 so please wait for my reply before ordering.

    2. Purchase ab110639 either by phone, fax, or online (www.abcam.com).

    3. Test it in Mouse.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab110639 turns out to be unsuitable for Mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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