Recombinant
RabMAb

Recombinant Anti-ERG antibody [EPR3864] (ab92513)

Rabbit recombinant monoclonal ERG antibody [EPR3864]. Validated in WB, IHC, Flow Cyt, ChIP, ICC/IF and tested in Mouse, Rat, Human. Cited in 73 publication(s). Independently reviewed in 12 review(s).

Overview

  • Product name
    Anti-ERG antibody [EPR3864]
    See all ERG primary antibodies
  • Description
    Rabbit monoclonal [EPR3864] to ERG
  • Host species
    Rabbit
  • Specificity
    This antibody also detects Fli-1.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ERG aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: P11308

  • Positive control
    • WB: Jurkat, HeLa and RAW 264.7 cell lysates; Rat brain and heart lysates. IHC-P: Human kidney, brain and prostate adenocarcinoma tissues; Fus A5 transgenic mouse prostate tissue. ICC/IF: Circulating tumor cells (CTCs) from a castrate-resistant prostate cancer (CRPC) patient; THP-1 cells. Flow Cyt: THP-1 cells.
  • General notes

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/100 - 1/250.

ICC/IF 1/100 - 1/250.
IHC-Fr Use at an assay dependent concentration. PubMed: 22860005
Flow Cyt Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 23817021

Target

  • Function
    Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
  • Involvement in disease
    Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
    Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Cellular localization
    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
    • D030036I24Rik antibody
    • Erg 3 antibody
    • Erg antibody
    • ERG/EWS fusion gene, included antibody
    • ERG/FUS fusion gene, included antibody
    • ERG/TMPSSR2 fusion gene, included antibody
    • ERG_HUMAN antibody
    • ERG1, included antibody
    • ERG2, included antibody
    • ets related antibody
    • ETS-related gene antibody
    • KCNH2 antibody
    • Oncogene ERG antibody
    • p55 antibody
    • TMPRSS2/ERG fusion antibody
    • transcriptional regulator ERG (transforming protein ERG) antibody
    • Transcriptional regulator ERG antibody
    • Transforming protein ERG antibody
    • v ets avian erythroblastosis virus E26 oncogene antibody
    • v ets avian erythroblastosis virus E26 oncogene related antibody
    • v ets erythroblastosis virus E26 oncogene homolog antibody
    • v ets erythroblastosis virus E26 oncogene like antibody
    • v ets erythroblastosis virus E26 oncogene like isoform 2 antibody
    • v-ets erythroblastosis virus E26 oncogene antibody
    • v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody
    • V-ets erythroblastosis virus E26 oncogene like (Avian), isoform CRA_e antibody
    see all

Images

  • All lanes : purified

    Lane 1 : rat brain lysate
    Lane 2 : rat heart lysate
    Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Functional characterization and detection of genetic alterations in GEDI-captured cells. The TMPRSS2:ERG fusion protein is detected in GEDI-captured circulating tumor cells (CTCs) from a castrate-resistant prostate cancer (CRPC) patient. PSMA-captured CTCs were stained on the device with ab92513. Representative examples of PSMA+/CD45− CTCs are shown, two of which are positive for ERG. Scale bars: 10 microns.

  • ERG and GSTP1 immunostainings of human prostate cancer samples using ab92513.

    Representative immunohistochemical images of prostate cancer samples are shown that were positive for ERG and negative for GSTP1 (A), positive for both ERG and GSTP1 (B), negative for both ERG and GSTP1(C), and negative for ERG and positive for GSTP1 (D). The internal staining control for ERG is the endothelium (arrows) and for GSTP1 the stromal and/or basal cells of normal prostate glands. N, normal prostate gland; S, Stroma; T, tumor gland. Scale bars equal 100μm

  • Immunohistochemical staining of paraffin embedded human kidney with purified ab92513 at a working dilution of 1/1000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Flow Cytometry analysis of THP-1 (human monocytic leukemia cell line) cells labeling ERG with purified ab92513 at 1:1000 dilution(1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • All lanes : Anti-ERG antibody [EPR3864] (ab92513) at 1/2000 dilution (purified)

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of THP-1 (human monocytic leukemia cell line) cells with purified ab92513 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92513 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • ab92513 staining ERG in Human brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 3% Horse serum) for 15 hours at 4°C. An undiluted HRP-conjugated Horse anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution (unpurified) + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

    Secondary
    HRP labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 55 kDa

  • Lanes 1 & 3 : Anti-ERG antibody [EPR3864] (ab92513) at 1/250 dilution (unpurified)
    Lanes 2 & 4 : Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution (unpurified)

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) Whole Cell Lysate
    Lanes 2-4 : Jurkat Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa


    Exposure time: 12 minutes
  • Immunohistochemical analysis of Fus A5 transgenic mouse prostate tissue, staining ERG with unpurified ab92513.

    Tissue sections were blocked in 20% goat serum, 2% BSA in PBS (GSB) for 40 min at room temperature, followed by an overnight incubation in primary antibody (1/25 in GSB) at 4°C. Following washes, the sections were incubated with a fluorescently tagged secondary antibody for 30 min at room temperature.

  • Immunohistochemical analysis of paraffin embedded Human Prostatic adenocarcinoma stage 3 tissue using unpurified ab92513 showing +ve staining.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:
  • Kim YH  et al. A MST1-FOXO1 cascade establishes endothelial tip cell polarity and facilitates sprouting angiogenesis. Nat Commun 10:838 (2019). Read more (PubMed: 30783090) »
  • Crist AM  et al. Vascular deficiency of Smad4 causes arteriovenous malformations: a mouse model of Hereditary Hemorrhagic Telangiectasia. Angiogenesis 21:363-380 (2018). IHC - Wholemount . Read more (PubMed: 29460088) »
See all 74 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (brain)
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Formaldehyde

Dr. 振洋 余

Verified customer

Submitted Mar 23 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (Aorta)
Specification
Aorta
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 30 2013

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Human Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 30 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Sample
Human Tissue sections (Aorta)
Specification
Aorta
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 30 2013

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 30 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 27 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lymph node)
Permeabilization
Yes - Yes - 0.05% Tween, 0.1% Triton
Specification
Lymph node
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 17 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (liver)
Specification
liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 01 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Antigen retrieval step
None
Permeabilization
Yes - PBS-Triton X100 0.2%
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Ricardo Santamaría

Verified customer

Submitted Apr 11 2017

Application
IHC - Wholemount
Sample
Mouse Tissue (whole retina)
Specification
whole retina

Mr. Nicolas Rama

Verified customer

Submitted Nov 17 2014

1-10 of 16 Abreviews or Q&A

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