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  1. Link

    erg-antibody-epr3864-ab92513.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
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RecombinantRabMAb

Recombinant Anti-ERG antibody [EPR3864] (ab92513)

  • Datasheet
  • SDS
Reviews (16)Q&A (4)References (158)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)
  • Western blot - Anti-ERG antibody [EPR3864] (ab92513)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864] (ab92513)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864] (ab92513)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)
  • Flow Cytometry (Intracellular) - Anti-ERG antibody [EPR3864] (ab92513)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)
  • Western blot - Anti-ERG antibody [EPR3864] (ab92513)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864] (ab92513)
  • Western blot - Anti-ERG antibody [EPR3864] (ab92513)
  • Western blot - Anti-ERG antibody [EPR3864] (ab92513)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)
  • OI-RD Scanning - Anti-ERG antibody [EPR3864] (ab92513)
  • Anti-ERG antibody [EPR3864] (ab92513)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3864] to ERG
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free Carrier Free HRP

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Overview

  • Product name

    Anti-ERG antibody [EPR3864]
    See all ERG primary antibodies
  • Description

    Rabbit monoclonal [EPR3864] to ERG
  • Host species

    Rabbit
  • Specificity

    This antibody also detects Fli-1.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, HeLa and RAW 264.7 cell lysates; Rat brain and heart lysates. IHC-P: Human kidney, brain and prostate adenocarcinoma tissues; Fus A5 transgenic mouse prostate tissue; Mouse brain tissue. ICC/IF: Circulating tumor cells (CTCs) from a castrate-resistant prostate cancer (CRPC) patient; THP-1 cells. Flow Cyt (intra): THP-1 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 8.90 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3864
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Stem Cells
    • Hematopoietic Progenitors
    • Intracellular Molecules
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine methylation

Associated products

  • Alternative Versions

    • HRP Anti-ERG antibody [EPR3864] (ab195514)
    • Alexa Fluor® 647 Anti-ERG antibody [EPR3864] (ab196149)
    • Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (ab196374)
    • Anti-ERG antibody [EPR3864] - BSA and Azide free (ab214796)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human ERG protein (ab185597)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P (3)
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/100 - 1/250.

ICC/IF (3)
Use a concentration of 1 µg/ml.

This product gave a positive signal in THP-1 (-ve: HCT116) fixed with 4% formaldehyde (10 min).

Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/100 - 1/250.

ICC/IF
Use a concentration of 1 µg/ml.

This product gave a positive signal in THP-1 (-ve: HCT116) fixed with 4% formaldehyde (10 min).

Flow Cyt (Intra)
Use at an assay dependent concentration.

Target

  • Function

    Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
  • Involvement in disease

    Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
    Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4.
  • Sequence similarities

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P11308 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2078 Human
    • Entrez Gene: 13876 Mouse
    • Entrez Gene: 170909 Rat
    • Omim: 165080 Human
    • SwissProt: P11308 Human
    • SwissProt: P81270 Mouse
    • Unigene: 473819 Human
    • Unigene: 164531 Mouse
    • Alternative names

      • Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
      • D030036I24Rik antibody
      • Erg 3 antibody
      • Erg antibody
      • ERG/EWS fusion gene, included antibody
      • ERG/FUS fusion gene, included antibody
      • ERG/TMPSSR2 fusion gene, included antibody
      • ERG_HUMAN antibody
      • ERG1, included antibody
      • ERG2, included antibody
      • ets related antibody
      • ETS-related gene antibody
      • KCNH2 antibody
      • Oncogene ERG antibody
      • p55 antibody
      • TMPRSS2/ERG fusion antibody
      • transcriptional regulator ERG (transforming protein ERG) antibody
      • Transcriptional regulator ERG antibody
      • Transforming protein ERG antibody
      • v ets avian erythroblastosis virus E26 oncogene antibody
      • v ets avian erythroblastosis virus E26 oncogene related antibody
      • v ets erythroblastosis virus E26 oncogene homolog antibody
      • v ets erythroblastosis virus E26 oncogene like antibody
      • v ets erythroblastosis virus E26 oncogene like isoform 2 antibody
      • v-ets erythroblastosis virus E26 oncogene antibody
      • v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody
      • V-ets erythroblastosis virus E26 oncogene like (Avian), isoform CRA_e antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)

      Formalin-fixed, paraffin-embedded mouse brain tissue stained for ERG using ab92513 at 1/200 dilution in immunohistochemical analysis. A horse radish peroxidase antibody was used as the secondary antibody.

      Antigen Retrieval: 40x; Proteinase K antigen retrieval - 15 min at 37 C

    • Western blot - Anti-ERG antibody [EPR3864] (ab92513)
      Western blot - Anti-ERG antibody [EPR3864] (ab92513)
      All lanes : purified

      Lane 1 : rat brain lysate
      Lane 2 : rat heart lysate
      Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864] (ab92513)
      Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864] (ab92513)

      ab92513 staining ERG in THP-1 cells, with negative expression in HCT116 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab92513 at 1 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864] (ab92513)
      Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864] (ab92513)Image from Kirby BJ et al., PLoS One. 2012;8(12):e83903. Fig 4.; doi: 10.1371/journal.pone.0035976. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Functional characterization and detection of genetic alterations in GEDI-captured cells. The TMPRSS2:ERG fusion protein is detected in GEDI-captured circulating tumor cells (CTCs) from a castrate-resistant prostate cancer (CRPC) patient. PSMA-captured CTCs were stained on the device with ab92513. Representative examples of PSMA+/CD45− CTCs are shown, two of which are positive for ERG. Scale bars: 10 microns.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)Image from Litovkin K et al., PLoS One. 2015;10(6):e0130651. Fig 5.; doi: 10.1371/journal.pone.0130651. Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

      ERG and GSTP1 immunostainings of human prostate cancer samples using ab92513.

      Representative immunohistochemical images of prostate cancer samples are shown that were positive for ERG and negative for GSTP1 (A), positive for both ERG and GSTP1 (B), negative for both ERG and GSTP1(C), and negative for ERG and positive for GSTP1 (D). The internal staining control for ERG is the endothelium (arrows) and for GSTP1 the stromal and/or basal cells of normal prostate glands. N, normal prostate gland; S, Stroma; T, tumor gland. Scale bars equal 100μm

    • Flow Cytometry (Intracellular) - Anti-ERG antibody [EPR3864] (ab92513)
      Flow Cytometry (Intracellular) - Anti-ERG antibody [EPR3864] (ab92513)

      Intracellular Flow Cytometry analysis of THP-1 (human monocytic leukemia cell line) cells labeling ERG with purified ab92513 at 1/1000 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)
      Immunohistochemical staining of paraffin embedded human kidney with purified ab92513 at a working dilution of 1/1000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-ERG antibody [EPR3864] (ab92513)
      Western blot - Anti-ERG antibody [EPR3864] (ab92513)
      All lanes : Anti-ERG antibody [EPR3864] (ab92513) at 1/2000 dilution (purified)

      Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 55 kDa
      Observed band size: 55 kDa



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864] (ab92513)
      Immunocytochemistry/ Immunofluorescence - Anti-ERG antibody [EPR3864] (ab92513)

      Immunofluorescence staining of THP-1 (human monocytic leukemia cell line) cells with purified ab92513 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab92513 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

      Alexa Fluor® 488 (ab196374) and Alexa Fluor® 647 (ab196149) conjugated versions are available for this clone.

    • Western blot - Anti-ERG antibody [EPR3864] (ab92513)
      Western blot - Anti-ERG antibody [EPR3864] (ab92513)
      Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution (unpurified) + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 55 kDa

    • Western blot - Anti-ERG antibody [EPR3864] (ab92513)
      Western blot - Anti-ERG antibody [EPR3864] (ab92513)
      Lanes 1 & 3 : Anti-ERG antibody [EPR3864] (ab92513) at 1/250 dilution (unpurified)
      Lanes 2 & 4 : Anti-ERG antibody [EPR3864] (ab92513) at 1/1000 dilution (unpurified)

      Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) Whole Cell Lysate
      Lanes 2-4 : Jurkat Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 55 kDa
      Observed band size: 55 kDa


      Exposure time: 12 minutes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (ab92513)

      Immunohistochemical analysis of paraffin embedded Human Prostatic adenocarcinoma stage 3 tissue using unpurified ab92513 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-ERG antibody [EPR3864] (ab92513)
      OI-RD Scanning - Anti-ERG antibody [EPR3864] (ab92513)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-ERG antibody [EPR3864] (ab92513)
      Anti-ERG antibody [EPR3864] (ab92513)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (158)

    Publishing research using ab92513? Please let us know so that we can cite the reference in this datasheet.

    ab92513 has been referenced in 158 publications.

    • Jiang Y  et al. Cerebral angiogenesis ameliorates pathological disorders in Nemo-deficient mice with small-vessel disease. J Cereb Blood Flow Metab 41:219-235 (2021). PubMed: 32151223
    • Velazquez JJ  et al. Gene Regulatory Network Analysis and Engineering Directs Development and Vascularization of Multilineage Human Liver Organoids. Cell Syst 12:41-55.e11 (2021). PubMed: 33290741
    • Nitzsche A  et al. Paladin is a phosphoinositide phosphatase regulating endosomal VEGFR2 signalling and angiogenesis. EMBO Rep 22:e50218 (2021). PubMed: 33369848
    • Ninchoji T  et al. eNOS-induced vascular barrier disruption in retinopathy by c-Src activation and tyrosine phosphorylation of VE-cadherin. Elife 10:N/A (2021). PubMed: 33908348
    • Andrade J  et al. Control of endothelial quiescence by FOXO-regulated metabolites. Nat Cell Biol 23:413-423 (2021). PubMed: 33795871
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    1-10 of 16 Abreviews

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ERG antibody [EPR3864]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (brain)
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More

    DR. 振洋 余

    Verified customer

    Submitted Mar 23 2017

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-ERG antibody [EPR3864]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step
    None
    Sample
    Human Tissue sections (Aorta)
    Specification
    Aorta
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 30 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-ERG antibody [EPR3864]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample
    Human Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 30 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ERG antibody [EPR3864]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Sample
    Human Tissue sections (Aorta)
    Specification
    Aorta
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 30 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-ERG antibody [EPR3864]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 30 2013

    ChIP abreview for Anti-ERG antibody [EPR3864]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (K562)
    Specification
    K562
    Detection step
    Other
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Read More

    Mr. Andrew Perez

    Verified customer

    Submitted Mar 24 2022

    Western blot abreview for Anti-ERG antibody [EPR3864]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Abcam user community

    Verified customer

    Submitted Aug 14 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-ERG antibody [EPR3864]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Embryonic heart)
    Permeabilization
    Yes - Tween-20
    Specification
    Embryonic heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
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    Abcam user community

    Verified customer

    Submitted Aug 10 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ERG antibody [EPR3864]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brown fat)
    Permeabilization
    Yes - 0.5% PBST
    Specification
    Brown fat
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 17 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-ERG antibody [EPR3864]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 27 2018

    1-10 of 16 Abreviews

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