Recombinant Anti-ERG antibody [EPR3864] - Mouse IgG2b (Chimeric) (ab214341)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [EPR3864] to ERG - Chimeric
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ERG antibody [EPR3864] - Mouse IgG2b (Chimeric)
See all ERG primary antibodies -
Description
Mouse monoclonal [EPR3864] to ERG - Chimeric -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat whole cell lysate (ab7899). IHC-P: FFPE human prostate cancer.
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General notes
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab92513). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3864 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
For unpurified use at 1µg/ml. |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples. See IHC antigen retrieval protocol. For unpurified use at 0.1-1µg/ml. |
Notes |
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WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). For unpurified use at 1µg/ml. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples. See IHC antigen retrieval protocol. For unpurified use at 0.1-1µg/ml. |
Target
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Function
Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. -
Involvement in disease
Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 2078 Human
- Entrez Gene: 13876 Mouse
- Entrez Gene: 170909 Rat
- Omim: 165080 Human
- SwissProt: P11308 Human
- SwissProt: P81270 Mouse
- Unigene: 473819 Human
- Unigene: 164531 Mouse
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Alternative names
- Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
- D030036I24Rik antibody
- Erg 3 antibody
see all
Images
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IHC image of ERG staining in a section of formalin fixed, paraffin embedded human prostate cancer. The section was pre-treated using pressure cooker heat mediated antigen retrieval with Tris-EDTA (pH9). The section was incubated with unpurified ab214341, 0.16 µg/ml, for 30 mins at room temperature followed by an incubation with a ready to use anti mouse HRP polymer system for 15 mins at room temperature. The section was counterstained with haematoxylin and mounted with permanent mounting media. As a negative control (inset) the same assay was performed on a section of formalin fixed, paraffin embedded, normal human prostate.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : Anti-ERG antibody [EPR3864] - Mouse IgG2b (Chimeric) (ab214341) at 1/1000 dilution (purified)
Lane 1 : Rat heart lysates
Lane 2 : Rat brain lysates
Lane 3 : Mouse brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Anti-ERG antibody [EPR3864] - Mouse IgG2b (Chimeric) (ab214341) at 1/5000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Lane 1 : Anti-ERG antibody [EPR3864] - Mouse IgG2b (Chimeric) (ab214341) at 1 µg/ml (unpurified)
Lane 2 : Anti-ERG antibody [EPR3864] (ab92513) at 1 µg/ml (unpurified)
All lanes : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Lane 2 : Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab214341 (lane 1) and ab92513 (lane 2) overnight at 4°C. Antibody binding was detected using an anti-mouse (lane 1) and anti-rabbit (lane 2) antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostatic hyperplasia tissue sections labeling ERG with purified ab214341 at 1:1000 dilution (1.39 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab214341 has been referenced in 2 publications.
- Warkala M et al. Cell-Extracellular Matrix Interactions Play Multiple Essential Roles in Aortic Arch Development. Circ Res 128:e27-e44 (2021). PubMed: 33249995
- Shen Y et al. Reduction of Liver Metastasis Stiffness Improves Response to Bevacizumab in Metastatic Colorectal Cancer. Cancer Cell 37:800-817.e7 (2020). PubMed: 32516590