Key features and details
- Rabbit polyclonal to ERGIC1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ERGIC1 antibody
See all ERGIC1 primary antibodies
DescriptionRabbit polyclonal to ERGIC1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: Human RT-4, U-251 MG and Human tonsil cell lysates IHC: Human liver, prostate, cerebral cortex and testis tissue ICC/IF: Human cell line U-251
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionPossible role in transport between endoplasmic reticulum and Golgi.
Sequence similaritiesBelongs to the ERGIC family.
Cellular localizationEndoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Cycles between the endoplasmic reticulum and the Golgi.
- Information by UniProt
- endoplasmic reticulum-golgi intermediate compartment 32 kDa protein antibody
- Endoplasmic reticulum-Golgi intermediate compartment protein 1 antibody
- ER-Golgi intermediate compartment 32 kDa protein antibody
ab121574, at a 1/25 dilution, staining ERGIC1 in paraffin-embedded Human liver tissue by immunohistochemistry.
ab121574, at a 1/25 dilution, staining ERGIC1 in paraffin-embedded Human prostate tissue by immunohistochemistry.
ab121574, at a 1/25 dilution, staining ERGIC1 in paraffin-embedded Human cerebral cortex tissue by immunohistochemistry.
ab121574, at a 1/25 dilution, staining ERGIC1 in paraffin-embedded Human testis tissue by immunohistochemistry.
All lanes : Anti-ERGIC1 antibody (ab121574) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121574 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121574 has not yet been referenced specifically in any publications.