Key features and details
- Rabbit polyclonal to ERGIC1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ERGIC1 antibody
See all ERGIC1 primary antibodies
DescriptionRabbit polyclonal to ERGIC1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: Transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag. ICC/IF: Human cell line U-2 OS IHC: Human colon and human duodenum tissue
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionPossible role in transport between endoplasmic reticulum and Golgi.
Sequence similaritiesBelongs to the ERGIC family.
Cellular localizationEndoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Cycles between the endoplasmic reticulum and the Golgi.
- Information by UniProt
- endoplasmic reticulum-golgi intermediate compartment 32 kDa protein antibody
- Endoplasmic reticulum-Golgi intermediate compartment protein 1 antibody
- ER-Golgi intermediate compartment 32 kDa protein antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121582 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121582 at a 1/50 dilution, staining ERGIC1 in glandular cells of paraffin-embedded Human colon tissue by immunohistochemistry.
All lanes : Anti-ERGIC1 antibody (ab121582) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : Lysate from transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
Immunohistochemical staining of Human duodenum shows strong cytoplasmic positivity using ab121582
ab121582 has been referenced in 2 publications.
- Wang F et al. Differential Expression and Significance of Endoplasmic Reticulum Golgi Intermediate Compartment 1 in Precancerous Gastric Lesions and Gastric Cancer. Am J Med Sci 355:228-234 (2018). PubMed: 29549924
- Wang FR et al. Aberrant DNA-PKcs and ERGIC1 expression may be involved in initiation of gastric cancer. World J Gastroenterol 23:6119-6127 (2017). ICC/IF . PubMed: 28970727