Anti-ERGIC1 antibody (ab121582)
Key features and details
- Rabbit polyclonal to ERGIC1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERGIC1 antibody
See all ERGIC1 primary antibodies -
Description
Rabbit polyclonal to ERGIC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: PGNFHVSTHS ATAQPQNPDM THVIHKLSFG DTLQVQNIHG AFNALGGADR LTSNPLASHD YIL, corresponding to internal sequence amino acids 127-189 of Human ERGIC1
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Positive control
- WB: Transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag. ICC/IF: Human cell line U-2 OS IHC: Human colon and human duodenum tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. | |
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. | |
| WB | Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
Possible role in transport between endoplasmic reticulum and Golgi. -
Sequence similarities
Belongs to the ERGIC family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Cycles between the endoplasmic reticulum and the Golgi. - Information by UniProt
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Database links
- Entrez Gene: 57222 Human
- SwissProt: Q969X5 Human
- Unigene: 509163 Human
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Alternative names
- endoplasmic reticulum-golgi intermediate compartment 32 kDa protein antibody
- Endoplasmic reticulum-Golgi intermediate compartment protein 1 antibody
- ER-Golgi intermediate compartment 32 kDa protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ERGIC1 antibody (ab121582)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121582 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121582)ab121582 at a 1/50 dilution, staining ERGIC1 in glandular cells of paraffin-embedded Human colon tissue by immunohistochemistry. -
Western blot - Anti-ERGIC1 antibody (ab121582)All lanes : Anti-ERGIC1 antibody (ab121582) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : Lysate from transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121582)Immunohistochemical staining of Human duodenum shows strong cytoplasmic positivity using ab121582
Protocols
Datasheets and documents
References (2)
ab121582 has been referenced in 2 publications.
- Wang F et al. Differential Expression and Significance of Endoplasmic Reticulum Golgi Intermediate Compartment 1 in Precancerous Gastric Lesions and Gastric Cancer. Am J Med Sci 355:228-234 (2018). PubMed: 29549924
- Wang FR et al. Aberrant DNA-PKcs and ERGIC1 expression may be involved in initiation of gastric cancer. World J Gastroenterol 23:6119-6127 (2017). ICC/IF . PubMed: 28970727
