Anti-ERGIC1 antibody (ab121582)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-ERGIC1 antibody
See all ERGIC1 primary antibodies -
Description
Rabbit polyclonal to ERGIC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: PGNFHVSTHS ATAQPQNPDM THVIHKLSFG DTLQVQNIHG AFNALGGADR LTSNPLASHD YIL, corresponding to internal sequence amino acids 127-189 of Human ERGIC1
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Positive control
- Human colon tissue; transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. | |
| WB | 1/250 - 1/500. |
Target
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Function
Possible role in transport between endoplasmic reticulum and Golgi. -
Sequence similarities
Belongs to the ERGIC family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Cycles between the endoplasmic reticulum and the Golgi. - Information by UniProt
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Database links
- Entrez Gene: 57222 Human
- SwissProt: Q969X5 Human
- Unigene: 509163 Human
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Alternative names
- endoplasmic reticulum-golgi intermediate compartment 32 kDa protein antibody
- Endoplasmic reticulum-Golgi intermediate compartment protein 1 antibody
- ER-Golgi intermediate compartment 32 kDa protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ERGIC1 antibody (ab121582)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121582 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121582)ab121582 at a 1/50 dilution, staining ERGIC1 in glandular cells of paraffin-embedded Human colon tissue by immunohistochemistry. -
Western blot - Anti-ERGIC1 antibody (ab121582)All lanes : Anti-ERGIC1 antibody (ab121582) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : Lysate from transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121582)Immunohistochemical staining of Human duodenum shows strong cytoplasmic positivity using ab121582
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wang F et al. Differential Expression and Significance of Endoplasmic Reticulum Golgi Intermediate Compartment 1 in Precancerous Gastric Lesions and Gastric Cancer. Am J Med Sci 355:228-234 (2018). Read more (PubMed: 29549924) »
- Wang FR et al. Aberrant DNA-PKcs and ERGIC1 expression may be involved in initiation of gastric cancer. World J Gastroenterol 23:6119-6127 (2017). ICC/IF . Read more (PubMed: 28970727) »
